荷叶生物碱对THP-1单核细胞源性巨噬细胞泡沫化及B类Ⅰ型清道夫受体表达的影响  

Effects of Nuciferine on SR-BⅠ Expression and Foam Formation of Human THP-1 Derived Macrophage

作　　者：常冠楠[1] 徐新[2] 张社兵[2] 

机构地区：[1]潍坊市益都中心医院,山东省青州市262500 [2]广东省粤北人民医院,广东省韶关市512000

出　　处：《中国动脉硬化杂志》2012年第7期611-615,共5页Chinese Journal of Arteriosclerosis

基　　金：广东省科技计划项目(2009B030801066);韶关科技计划项目[韶科(卫)2009-01]

摘　　要：目的观察荷叶生物碱对氧化型低密度脂蛋白(ox-LDL)所致人THP-1巨噬细胞泡沫化及B类Ⅰ型清道夫受体(SR-BⅠ)表达的影响,探讨荷叶生物碱抗动脉粥样硬化的可能机制。方法建立THP-1源性泡沫细胞模型后,采用不同剂量荷叶生物碱(0、25、50、100 mg/L)分组共孵育24 h。油红O染色观察细胞内脂质蓄积情况;分别用荧光定量PCR、Wesetern Blot检测细胞中SR-BⅠmRNA水平和蛋白水平。结果与空白对照组比较,ox-LDL对细胞内脂滴积聚呈浓度依赖性;且细胞SR-BⅠ的mRNA水平(分别为1.00±0.00、0.53±0.04、0.30±0.04和0.18±0.03)及蛋白表达(分别为1.00±0.00、0.62±0.10、0.39±0.08和0.22±0.05)呈浓度依赖性降低(P<0.01);加入荷叶生物碱干预后,细胞内脂滴随荷叶生物碱浓度的增加而减少,细胞SR-BⅠmRNA水平(分别为0.32±0.02、1.07±0.02、1.28±0.03和1.49±0.03)及蛋白表达(分别为0.28±0.03、1.06±0.02、1.32±0.03和1.41±0.02)浓度依赖性增加(P<0.01)。结论荷叶生物碱上调人THP-1单核细胞源性泡沫细胞SR-BⅠ的表达,促进细胞内胆固醇流出,可能是荷叶生物碱抗动脉粥样硬化的机制之一。Aim To investigate the effect of nuciferine on scavenger receptor class B type Ⅰ（SR-BⅠ） expression and foam formation in human THP-1 derived macrophage induced by oxidized low density lipoprotein（ox-LDL）,and probe the possible mechanism of nuciferine in anti-atherosclerosis.Methods After the establishment of monocyte lines THP-l-derived foam cell,these foam cells were treated with nuciferine at different concentrations（0,25,50,100 mg/L）for 24 h.The effect of nuciferine on foam cell was observed by oil red O staining;the levels of SR-BⅠ mRNA and protein were measured by RT-PCR and Western Blot,respectively.Results Compared with control group,lipid droplets was increased while mRNA（1.00±0.00,0.53±0.04,0.30±0.04,0.18±0.03）and protein（1.00±0.00,0.62±0.10,0.39±0.08,0.22±0.05）expression of SR-BⅠ were downregulated in ox-LDL groups in a dose-dependent manner（P0.01）;after the intervention of nuciferine,lipid droplets was decreased while mRNA（0.32±0.02,1.07±0.02,1.28±0.03,1.49±0.03）and protein（0.28±0.03,1.06±0.02,1.32±0.03,1.41±0.02） expression of SR-BⅠ were upregulated in a dose-dependent manner（P0.01）.Conclusion Nuciferine upregulates SR-BⅠ expression in THP-1 derived macrophages,lessen the degree of foam formation.We probe that this may be the possible mechanism of nuciferine in anti-atherosclerosis.

关 键 词：荷叶生物碱 单核细胞株THP-1 氧化型低密度脂蛋白 B类Ⅰ型清道夫受体 

分 类 号：R5[医药卫生—内科学]