青蒿琥酯对变应性接触性皮炎的免疫调节作用及其机制探讨  

Immunoregulatory effect of artesunate on allergic contact dermatitis and its mechanism

作　　者：李覃[1,2] 陈虹[2,3] 刘晓光[3] 周雅逊 白淑芳[3] 

机构地区：[1]天津武警后勤学院免疫学教研室,天津300162 [2]天津武警后勤学院天津市职业与环境危害生物标志物重点实验室,天津300162 [3]天津武警后勤学院生药学教研室,天津300162

出　　处：《药学学报》2012年第7期884-889,共6页Acta Pharmaceutica Sinica

基　　金：中国博士后科学基金面上项目(20090461498);中国博士后科学基金特别资助项目(201104796);天津市应用基础与前沿技术研究计划(11JCYBJC14600)

摘　　要：青蒿琥酯(AST)的免疫调节效应已被认可,但其具体作用的机制尚不明确。本研究通过变应性接触性皮炎(ACD)小鼠模型,初步探讨AST对ACD的免疫调节作用及可能的分子机制。采用药效学分析以及HE染色、半定量RT-PCR、Western blotting等方法分别检测AST对ACD相关细胞因子、转录因子及信号分子表达的影响。结果显示,局部应用AST能够抑制ACD小鼠耳肿胀度、脾指数、炎症细胞浸润以及IFN-γ、T-bet的表达,同时减弱NF-κB p65的表达。结果表明,AST能够通过抑制炎症反应和ACD小鼠免疫功能,调节机体的Th1/Th2失衡发挥作用,NF-κB可能是其作用靶点之一。This study is to elucidate the immunoregulation mechanisms of artesunate （AST） on allergic contact dermatitis （ACD）. Pharmacodynamies analyses, HE staining, semi-quantitative RT-PCR and Western blotting were used to explore the effects of AST on the related cytokines, transcription factor and signaling molecule of ACD respectively. The results indicated that topical administration of AST not only reduced the increase of ear swelling, spleen index and inflammatory cells infiltration in ACD mice, but also inhibited remarkably the expression of IFN-γ, T-bet and NF-kB p65. It＇s suggested that AST could exhibit suppressive effects on inflammatory response and immune function of ACD, which indicates the possibility of developing AST as a novel immunoregulatory agent in the treatment of ACD and other immune-related diseases.

关 键 词：变应性接触性皮炎 青蒿琥酯 免疫平衡 NF-ΚB 

分 类 号：R967[医药卫生—药理学]