金柴抗病毒胶囊对IFITM3蛋白表达的影响  

Effects of Jin Chai antiviral capsule on IFITM3 expression in mice

作　　者：钟菊迎[1] 崔晓兰[1] 时宇静[1] 郭姗姗[1] 刘颖[1] 刘方舟[1] 高英杰[1] 金亚宏[1] 曹洪欣[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国中医科学院,北京100700

出　　处：《药学学报》2012年第7期904-908,共5页Acta Pharmaceutica Sinica

基　　金：国家"重大新药创制"专项资助项目(2009ZX09301-005-013)

摘　　要：探讨金柴抗病毒胶囊对甲型H1N1流感病毒FM/1/47株感染小鼠肺炎模型的治疗作用机制。滴鼻感染正常小鼠,建立流感病毒肺炎模型并进行治疗性给药。采用实时荧光定量PCR法、Western blot在流感病毒感染后第1、3、5、7天检测小鼠肺组织中病毒载量、干扰素诱导跨膜蛋白的动态变化。实验结果显示,金柴抗病毒胶囊可降低肺组织病毒载量,其中大、中、小3个剂量组在病毒感染正常小鼠后各检测时间点均能明显降低肺组织中病毒载量的表达(P<0.05,P<0.01)。金柴抗病毒胶囊可上调干扰素诱导跨膜蛋白相对表达量。与模型对照组比较,其中大剂量在各个时间点均明显高表达(P<0.05,P<0.01),中剂量在第3、5天具有明显高表达(P<0.05);小剂量组在第3天表现显著高表达(P<0.05)。实验结果表明:金柴抗病毒胶囊可能通过上调流感病毒感染小鼠肺组织内干扰素诱导跨膜蛋白表达量来发挥抗流感病毒的作用。This study is to investigate the treatment of Jin Chai antiviral capsule for influenza virus FM1/47 （H1N1） infection. The model of pneumonia was established by dropping influenza virus into the nose of normal mice, real-time PCR and Western blot technique were used to detect the virus load and the interferoninducible transmembrane protein3 （IFITM3） in lung of mice at the 1st day, 3rd day, 5th day and 7th day after affected. The results showed that Jin Chai antiviral capsule in large, middle, small dose groups can decrease virus load significantly at each time point, after being affected （P〈0.05, P〈0.01）, Jin Chai antiviral capsule can increase the interferoninducible transmembrane protein3 in lung of mice, large dose groups are significantly higher in expression of IFITM3 compared with model group at each time point （P〈0.05, P〈0.01）. Middle dose groups are significantly higher in expression of IFITM3 compared with model group at the 3th day and the 5th day （P〈0.05）, small dose groups are significantly higher in expression of IFITM3 compared with model group at the 3th day （P〈 0.05）. It can be concluded that Jin Chai antiviral capsule exerts antiviral effects against influenzavirus by raised expression of IFITM3.

关 键 词：金柴抗病毒胶囊 甲型流感病毒 病毒载量 干扰素诱导跨膜蛋白 

分 类 号：R284[医药卫生—中药学]