一测多评法测定五味子中4种木脂素类成分的含量  

Simultaneous quantitative analysis of four lignanoids in Schisandra chinensis by quantitative analysis of multicomponents by single marker

作　　者：贺凤成 李守信 赵志全 董金平 刘武占 苏瑞强 

机构地区：[1]鲁南制药集团股份有限公司 [2]中药制药共性技术国家重点实验室,山东临沂276006

出　　处：《药学学报》2012年第7期930-933,共4页Acta Pharmaceutica Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2010CB735604)

摘　　要：建立五味子中4个木脂素类化合物一测多评方法学考察模式,验证该方法在五味子质量控制中应用的可行性和技术适应性。采用HPLC以五味子醇甲为内参物,建立五味子酯甲、五味子甲素和五味子乙素与五味子醇甲的相对校正因子,并进行含量计算,实现一测多评。同时采用外标法测定13批五味子药材中4个成分的含量,比较计算值与实测值的差异,验证一测多评法的准确性。结果表明,建立的校正因子重现性良好,采用校正因子计算的含量值与外标法实测值之间无显著差异。表明在缺少对照品的情况下,以外标法测定五味子醇甲含量,利用相对校正因子实现对五味子酯甲、五味子甲素和五味子乙素的测定准确可行,此方法可以用于五味子中木脂素类成分的定量评价研究。The aim of the study is to establish a new method of quality evaluation and validate its feasibilities by the simultaneous quantitative assay of four lignanoids in Schisandra chinensis. A new quality evaluation method, quantitative analysis of multi-components by single marker （QAMS）, was established and validated with Schisandra chinensis. Four main lignanoids, schisandrin, schisantherin A, deoxyschizandrin and y-schizandrin, were selected as analytes and schisandrin as internal reference substance to evaluate the quality. Their contents in 13 different batches of samples, collected from different bathes, were determined by both external standard method and QAMS. The method was evaluated by comparison of the quantitative results between external standard method and QAMS. No significant differences were found in the quantitative results of four lignanoids in 13 batches of S. chinensis determined by external standard method and QAMS. QAMS is feasible for determination of four lignanoids simultaneously when some authentic standard substances were unavailable, and the developed method can be used for quality control orS. chinensis.

关 键 词：相对校正因子 五味子 木脂素 HPLC 

分 类 号：R917[医药卫生—药物分析学]