复合疲劳对大鼠不同组织矿物质代谢影响研究(英文)  

Effects of Induced Fatigue on the Levels of Selected Essential Elements in the Blood and Tissues of Rats

作　　者：宫献文[1] 赵鑫[1] 李娅[1] 王文岚[1] 张学思[2] 郭庆军[1] 谢小萍[1] 任杰[1] 李金声[1] 常耀明[1] 

机构地区：[1]第四军医大学航空航天医学系卫生与卫生勤务学教研室,陕西西安710032 [2]第四军医大学科研部,陕西西安710032

出　　处：《现代生物医学进展》2012年第16期3012-3018,共7页Progress in Modern Biomedicine

基　　金：National Science and Technology Major Projects(2008ZXJ9009-005)~~

摘　　要：目的:研究复合疲劳大鼠血液、肌肉、肝脏和脑中矿物元素代谢变化的影响。方法:将30只大鼠随机分为正常对照组、食物限制组和复合疲劳组。经过5天的实验干预后,提取动物的血液、腓肠肌、肝脏和脑,并利用原子吸收分光光度法测量各组织中的钾(K)、钙(Ca)、镁(Mg)、铁(Fe)、锌(Zn)和铜(Cu)。结果:相对正常对照组和食物限制组,复合疲劳大鼠的肌肉、肝脏和脑中的K(P<0.01)和肝脏中的Fe(P<005)明显升高,血液中的Cu(与正常对照组比较P<0.01,与食物限制组比较P<0.05)明显下降;与对照组相比,复合疲劳大鼠的血液中的K明显升高(P<0.05),血液中的Mg和Zn(P<0.05),肌肉中的Ca、Mg和Zn(PCa<0.05,PMg<0.05,PZn<0.01),肝脏中的Ca和Zn(PCa<0.01,PZn<0.05),以及脑中的Fe、Mg和Zn(PFe<0.05,PMg<0.05,PZn<0.01)明显降低。结论:在复合疲劳状态下,大鼠血液、肌肉、肝脏和脑中的K、Ca、Mg、Fe、Zn和Cu代谢发生变化,可能在疲劳的发生与缓解中发挥作用。Objective： To identify the changes of some selected elements in blood,skeletal muscle,liver,and brain in a rat model with complex fatigue.Methods： 30 rats in the experiment were randomly divided into three groups： the control group,the food-restricted group,the complex fatigued group.Blood and tissue samples were collected at the end of the 5-day and levels of potassium（K）,calcium（Ca）,magnesium（Mg）,iron（Fe）,zinc（Zn）,and copper（Cu） were measured with atomic absorption spectrophotometer.Results： Compared with that in the control and food-restricted group,the concentrations of potassium in the skeletal muscle,liver,and brain（P0.01）,the concentration of iron in the liver（P005）,were increased in the complex fatigued group,and the concentration of copper in the blood（P0.01,to the control,P0.05,to the food-restricted） decreased in animals with complex fatigue;compared with that in the control group,the concentration of potassium in the blood was increased in the complex fatigued group（P0.05）,the concentrations of magnesium and zinc in the blood（P0.05）,the concentrations of calcium,magnesium,and zinc in the skeletal muscle（PCa0.05,PMg0.05,PZn0.01）,the concentrations of calcium,and zinc in the liver（PCa0.01,PZn0.05）,and the concentrations of iron,magnesium,and zinc in the brain（PFe0.05,PMg0.05,PZn0.01） were decreased in animals with complex fatigue.Conclusions： The metabolism of potassium,calcium,magnesium,iron,zinc,and copper in blood,skeletal muscle,liver,and brain changed differently in rats with complex fatigue,which indicated that these minerals may play some important role in the occurrence and relieving of fatigue.

关 键 词：疲劳 矿物质 微量元素 原子吸收分光光度法 

分 类 号：Q95-33[生物学—动物学] R591.1[医药卫生—内科学]