尿激酶型纤溶酶原激活物及其Ⅰ型抑制剂mRNA在实验性肌炎肌肉组织中的表达和意义  

Expression and significance of gene mRNA of urokinase type plasminogen activator and its inhibitor 1 type in muscle of exprimental autoimmune myositis guinea pigs

作　　者：段枫[1] 

机构地区：[1]海军总医院神经内科,北京100048

出　　处：《武警医学》2012年第6期508-511,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨尿激酶型纤溶酶原激活物(urokinase type plasmin-ogen activator,uPA)及其Ⅰ型抑制剂(plasminogenactivator inhibitor,PAI-1)mRNA在豚鼠实验性自身免疫性肌炎(experimental autoimmune myositis,EAM)肌肉中的表达及其意义。方法利用兔骨骼肌肌球蛋白加完全弗氏佐剂多次免疫豚鼠,同时联合腹腔注射百日咳毒素,制成EAM模型,观察其临床表现、血CK、肌肉病理的改变;用RT-PCR方法检测11例EAM组模型成功豚鼠肌肉uPA和PAI-1基因mRNA的表达,以10例正常豚鼠的肌肉作为对照组。结果 (1)EAM组肌肉中uPA、PAI-1基因mRNA的表达均较正常组显著增高(P<0.01);(2)肌肉中uPA与PAI-1基因mRNA表达的比例在正常组和EAM组无统计学差异(P<0.01)。结论 uPA、PAI-1可能参与了EAM的发病机制。Objective To study the expression and significance of gene mRNA of urokinase type plasminogen activator and its inhibitor 1type （PAI - 1 ） in the muscle of exprimental autoimmune myositis （EAM） guinea pigs. Methods The EAM model was es- tablished by repeatedly immunizating guinea pigs with rabbit skeletal muscle myosin plus Freund$ complete adjuvant and giving these pigs a celiac injection of pertussis toxin. The changes in clinical symptoms, serum ereatine kinase and pathology were observed. The expression level of uPA and PAI- 1 in the skeletal muscle was detected by RT - PCR and compared between EAM group （ n = 11 ） and normal group （ n = 10）. Results （ 1 ） The level of expression of the uPA and PAI - 1 mRNA in the muscle increased significantly in EAM group, compared with normal group（ P 〈 0.01 ）. （ 2 ） There was no difference in the ratio between expression of the uPA and PAI - 1 mRNA in the muscle tissues of EAM group and normal group （ P 〈 0.01 ）. Conclusions uPA and PAI- 1 may contribute to the pathogenesis of EAM.

关 键 词：实验性自身免疫性肌炎 尿激酶型纤溶酶原激活物 纤溶酶原激活物抑制剂 豚鼠 

分 类 号：R744.5[医药卫生—神经病学与精神病学]