二硫化碳对雄性大鼠下丘脑-垂体-性腺轴分泌功能的影响及NO的干预作用  

Effect of CS_2 on the secretory function of hypothalamus-pituitary-gonad axis in male rats and the intervention of NO

作　　者：马纪英[1,2] 周义军[1,3] 张振[1] 郭寅生[1] 王为[1] 董煜[1] 季佳佳[1,2] 丁情[1] 陈国元[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系,湖北武汉430030 [2]深圳市疾病预防与控制中心环境卫生科,广东深圳518055 [3]上海交通大学公共卫生学院,上海200052

出　　处：《国外医学（医学地理分册）》2012年第2期101-105,共5页Foreign Medical Sciences：Section of Medgeography

基　　金：国家自然科学基金资助项目(No.30471442;30872091;81172637)

摘　　要：目的探讨二硫化碳(CS_2)对雄性大鼠下丘脑-垂体-性腺轴(hypothalamic-pituitary-gonad axis,HPGA)分泌功能的影响,同时观察一氧化氮(NO)对其的干预作用。方法清洁级SD雄性大鼠36只随机分为6组,以不同浓度CS_2(0、50、250、1 250 mg/m^3)静式吸入染毒,共10周,另设CS2(1 250 mg/m^3)+SNP(5 mg/kg)组和CS_2(1 250 mg/m^3)+L-NMMA(2 mg/kg)组,染毒结束前10 d,对CS2高剂量染毒的3个组分别进行腹腔内注射生理盐水(NS)、SNP及L-NMMA,染毒结束后,测定大鼠血清、下丘脑、垂体、睾丸中NOS活性及NO含量,采用放射免疫法检测血清Gn-RH、FSH、LH、T的含量。结果 (1)随着CS_2染毒剂量的增加,大鼠血清、下丘脑、垂体、睾丸中NOS与iNOS活性、NO含量与对照组相比较均有不同程度的降低和减少;加入SNP后,NOS活性、iNOS活性均有所增高,与高剂量组相比较差异具有统计学意义(P<0.05),NO含量均有所增加,但差异无统计学意义(P>0.05);加入L-NMMA后NOS活性、iNOS活性降低、NO含量减少,但与高剂量组比较无统计学意义(P>0.05)。(2)随着CS_2染毒剂量的增加,血清中GnRH、LH的含量均有所下降,FSH含量随着染毒剂量的增加而增加,T有先升高后降低的趋势。加入SNP后,血清中GnRH、LH及T含量均有所升高,且与高剂量组比较具有统计学意义(P<0.05),FSH含量变化不明显(P>0.05);加入L-NMMA后,血清中GnRH、LH及T含量均有所降低,FSH含量变化不明显,与高剂量组比较均无统计学意义(P>0.05)。结论 (1)CS_2染毒对大鼠体内NO含量有一定的影响;(2)CS_2染毒能影响大鼠HPGA分泌的性激素有影响,NO对其具有一定的干预作用。Objective To study the effects of carbon disulfide on the secretory function of hypothalamus-pitui- tary-gonad axis in male rats and the intervention of NO. Methods 36 SD male rats were randomly divided into six groups. It took 10-week for the rats to expose to CS2 in different concentrations （0,50,250,1 250 mg/m3） , 10 days before the end of CS2 exposure, rats inhaled 1 250 mg/m3 CSz were injected with NS, SNP （5 mg/kg） or L-NMMA （2 mg/kg） everyday respectively. CS2 （1 250 mg/m3） with SNP （5 mg/kg, intraperitoneal injection） group and L- NMMA（2 mg/kg, intraperitoneal injection）group. After 10-week of CS2-exposure, the NOS activity and NO levels in serum, hypothalamus, pituitary, testis and hormones （GnRH, FSH, LH and T in serum） were measured. Results （1） After exposure to CS2, NOS and iNOS activity, NO content in serum, hypothalamus, pituitary, testis were lowered and reduced at different levels; after SNP was added, NOS activity, iNOS activity, NO levels have increased, compared with the high concentration group（P〈0.05）; after added L-NMMA, NOS activity, iNOS activity and NO content decreased,but it was not significant （P〉0.05）. （2） With the increasing concentration of CS2, GnRH, LH levels are decreased, FSH increased, T levels was first increased and then decreased. Added SNP, GnRH, LH and T contents were increased compared with the high dose group （P〈0.05）, FSH levels did not change significantly （P〉0. 05） ;after L-NMMA was added, GnRH, LH andT levels were decreased, FSH levels did not change significantly（P〉0.05）. Conclusion （1） CSz has a certain impact on NO levels in rats; （2） The sex hormone secretion of hypothalamus-pituitary-gonad axis in male rats can be affected by CS2, NO can interfere with the process to some extent.

关 键 词：二硫化碳 下丘脑-垂体-性腺轴 一氧化氮 

分 类 号：R135.1[医药卫生—劳动卫生]