丙泊酚对BV-2小胶质细胞活化的影响  

Effect of propofol on lipopolysaccharides-induced activation of BV-2 microglia cells

作　　者：宿明艳[1] 桂波[1] 钱燕宁[1] 

机构地区：[1]南京医科大学第一附属医院麻醉科,江苏省210029

出　　处：《江苏医药》2012年第12期1365-1367,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金(81070276);教育部博士点基金(20103234110008)

摘　　要：目的探讨丙泊酚对脂多糖(LPS)诱导的BV-2小胶质细胞活化的影响及其可能机制。方法体外培养小鼠BV-2小胶质细胞,随机分为LPS 1μg/ml组(A组)、丙泊酚30μM组(B组)、LPS 1μg/ml+丙泊酚30μM组(C组)和空白对照组(D组)。采用RT-PCR检测各组细胞中IL-1β和TNF-αmRNA表达量,Western blot检测总糖原合成酶激酶-3β(GSK-3β)和磷酸化GSK-3β(p-GSK-3β)的蛋白表达水平。结果 A、C组IL-1β、TNF-α及p-GSK-3表达量均较D组明显增加(P<0.05或P<0.01)。与A组相比,C组IL-1β和TNF-αmRNA表达量降低,而p-GSK-3β蛋白表达量增加(P<0.05)。结论丙泊酚30μM能在体外减轻LPS诱导的BV-2小胶质细胞释放IL-1β和TNF-α的水平,此作用可能与抑制GSK-3β活性有关。Objective To investigate the effect and possible mechanism of propofol on lipopolysaccharides（LPS）-induced activation of BV-2 microglia cells. Methods BV-2 microglia cells were cultured in vitro and randomly divided into four groups of A （ treated with LPS 1 μg/ml）, B （treated with propofol 30 μM）, C（treated with LPS 1 μg/ml plus propofol 30 μM） and D（biank control）. The mRNA expressions of IL-1β and TNF-α were detected by RT-PCR. The protein expressions of total glycogen synthase kinase-313（GSK 3β） and phosphorylation of GSK 3β（p-GSK-3β） were analyzed by Western blot. Results The expressions of IL-1β ,TNF-a and p-GSK-3βin groups of A and C were significantly higher than those in group D（P〈0. 05 or P〈0. 01）. Compared with group A, the mRNA expressions of IL-1β〈 and TNF-a were decreased, while protein expression of p-GSK-3β was increased in group C （P〈0. 05 ）. Conclusion Propofol 30 μM down-regulates LPS-induced expressions of IL-1β and TNF-a in BV-2 microglia cells in vitro, which is possibly related with inhibiting the activation of GSK-3β.

关 键 词：丙泊酚 小胶质细胞 糖原合成酶激酶-3Β 

分 类 号：R971[医药卫生—药品]