降钙素基因相关肽在舌下神经切断后大鼠舌下神经核中表达的变化  

Changes of CGRP expression in hypoglossal nucleus of rats after hypoglossal nerver transection

作　　者：李建国[1] 孟壮志[1] 刘海英[1] 李迪[1] 田耕[1] 毕伏龙[1] 

机构地区：[1]内蒙古民族大学人体解剖教研室,028041

出　　处：《江苏医药》2012年第12期1399-1400,共2页Jiangsu Medical Journal

摘　　要：目的研究降钙素基因相关肽(CGRP)在舌下神经损伤后大鼠舌下神经核中表达的变化。方法 36只SD大鼠行左侧舌下神经切断术,而右侧在相应部位仅行舌下神经游离术,分别于术后3、7、14、21、28、35d各取6只大鼠含舌下神经核团脑干部分,用免疫组化法及图像分析技术观察大鼠两侧舌下神经核中CGRP表达的变化。结果 CGRP分布于正常大鼠舌下神经各亚核;术后不同时间点,左侧舌下神经核中CGRP表达较右侧明显增加(P<0.05)。结论周围性舌下神经损伤导致CGRP在舌下神经核中的表达增加,提示CGRP在舌下神经再生修复过程中发挥调节作用。Objective To investigate the changes of calcitoriin gene-related peptide （CGRP） expression in the hypoglossal nucleus of rats after hypoglossal nerver transection. Methods The left sides of thirty-six 813 rats were operated with hypoglossal nerve transection, while the corresponding right sides were only done with hypoglossal nerve dissociation. On the 3^rd , 7^th, 14^th , 21^st, 28^th, and 35^th day after operation,the brain stems containing hypoglossal nucleus were taken out, respectively. The changes of CGRP expression in the left and right hypoglossal nucleus were detected by immunohistochemistry and image analysis technique. Results CGRP was distributed in the hypoglossal nucleus of normal rats, and the CGRP expression on the left side was significantly higher than that on the right side at different times after operation（P〈0. 05）. Conclusion The injury of peripheral hypoglossal nerve leads to an increase of CGRP expression in the hypoglossal nucleus, indicating that CGRP plays a regulatory role in the regeneration process of the injured hypoglossal

关 键 词：舌下神经 降钙素基因相关肽 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]