虾夷扇贝脓胞病病原的分离、鉴定与致病性  

Isolation, identification and pathogenicity of Vibrio chagasii from Patinopecten yessoensis

作　　者：滕炜鸣[1] 李文姬[1] 张明[1] 于佐安[1] 李石磊[1] 刘项峰[1] 李华琳[1] 付成东[1] 

机构地区：[1]辽宁省海洋水产科学研究院,辽宁省应用海洋生物技术开放实验室,辽宁省海洋水产分子生物学重点实验室,辽宁大连116023

出　　处：《水产学报》2012年第6期937-943,共7页Journal of Fisheries of China

基　　金：国家海洋公益性行业科研专项(200805037);辽宁省农业科技特派项目(2008203004)

摘　　要：开展对大连市长海县附近养殖海域近几年夏季出现的大规模虾夷扇贝死亡现象的研究,能为虾夷扇贝养殖过程中的疾病防治提供基础理论支持。以患病虾夷扇贝为研究对象,通过对病变组织进行细菌分离、提纯培养,并采用人工感染试验确定虾夷扇贝脓胞病的病原性质;通过生理生化特性、16SrRNA基因部分序列和药物敏感性测定,对虾夷扇贝脓胞病进行了鉴定。结果表明,从患病组织中分离出的其中一株细菌经人工感染试验证实为虾夷扇贝脓胞病的病原菌,在水温19℃、注射浓度为1.09×105CFU/mL的条件下,该菌有较强的致病性。通过对该病原菌的生理生化特性测定和16SrRNA基因部分序列分析,确定虾夷扇贝脓胞病的病原为查氏弧菌。In recent years cultured sea scallop （Patinopecten yessoensis） dead on a large-scale in seas near Changhai County, Dalian, China, in summer. The main symptom is the abscess on adductor muscle. The transmission experience was improved to indicate that a bacterium isolated from natural infected focus, named XJ02 displayed intense pathological activity to scallops, and the symptom is the same to natural intention. The bacterium XJ02 identification test through morphological specificity, physical and chemical properties and 16S rRNA gene sequence analysis showed the bacterium is Vibrio chagasii. There has been no any research on the pathogenicity of V. chagasii. This article for the first time to find a more compre-hensive’s vibrio physicochemical properties of research. The dose-and temperature-dependent test indi-cated that the bacterium had the most pathogenicity at 19 ℃ while the concentration was 1.09×105 CFU/mL. The medicine sensitivity analysis that？s to check Ampicillin, Benzylpenicillin, Ciproflxacin etc is sensitive during 20 drugs sensitive test, to Tetracycline, Doxycycline is not sensitive element.

关 键 词：虾夷扇贝 查氏弧菌 脓胞病 

分 类 号：S944.3[农业科学—水产养殖]