扩展莫尼茨绦虫不同体节翻译延伸因子和引发酶mRNA转录水平研究  

mRNA Transcription of Translation Elongation Factor and Primase in Different Segments of Moniezia expansa

作　　者：王正荣[1,2] 薄新文[1,2] 赵文娟[1,2] 康立超[1,2] 

机构地区：[1]新疆农垦科学院,石河子832000 [2]新疆绵羊重点繁育实验室,石河子832000

出　　处：《中国寄生虫学与寄生虫病杂志》2012年第3期250-252,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家重点基础研究发展计划(973计划)项目(No.2006CB708512);兵团杰出青年创新资金专项(No.2011CD005);兰州兽医研究所家畜疫病病原生物学国家重点实验室2011年度开放基金(No.SKLVEB2011KFKT008)~~

摘　　要：本研究以β微管蛋白基因作为内参基因,采用实时荧光定量PCR方法检测该基因在扩展莫尼茨绦虫(Moniezia expansa)4个不同发育阶段,即头节、幼节、成节和孕节中的表达差异。标准曲线分析显示,翻译延伸因子、引发酶基因和β微管蛋白基因标准质粒实时定量扩增Ct值与标准质粒的浓度均呈良好的线性关系,相关系数(R2)均大于0.99。同时实验结果显示,翻译延伸因子和引发酶基因在虫体各发育阶段中的转录水平存在差异。其中,翻译延伸因子和引发酶基因在扩展莫尼茨绦虫头节和幼节的转录水平均较高,提示这2个基因可能在头节和幼节的发育中有重要作用。A SYBR green real-time fluorescent quantitative PCR assay was developed for detection of Moniezia expansa mRNA with its beta-tubulin as an internal control. The results showed a good linear relationship （〉0.99）between the Ct value and the concentration of positive plasmid for each gene from scolex and various proglottids. Real-time PCR showed that the expression abundance of translation elongation factor and primase was different. In conclusion, the tran- scription level of translation elongation factor and primase was high in both scolex and immature segment, suggesting that they may olav a role in the develonment of scolex and immature segment.

关 键 词：扩展莫尼茨绦虫 翻译延伸因子 引发酶 mRNA转录水平 

分 类 号：R531.11[医药卫生—内科学]