腹部开放伤合并海水浸泡动物感染实验研究  

Experimental Study on Animal Infection after Seawater Immersion of Open Abdominal Wound

作　　者：韩善桥[1] 虞积耀[1] 王大鹏[1] 刘瑾红[1] 

机构地区：[1]中国人民解放军海军总医院,北京100048

出　　处：《实用预防医学》2012年第6期912-913,922,共3页Practical Preventive Medicine

基　　金：全军医药卫生科研基金课题(编号:06D004)

摘　　要：目的了解腹部开放伤合并海水浸泡动物细菌感染的特点。方法制作兔腹部开放伤动物感染实验模型并分成对照组和实验组。实验组致伤后放海水浸泡20 min,对照组致伤后不浸泡。用手工法和全自动微生物分析仪进行细菌学鉴定,用微生物动态快速测定系统检测血浆内毒素含量,全血白细胞数用显微镜计数法。结果实验组与对照组相比,12 h后细菌数量显著增加,主要细菌为大肠埃希菌、奇异变形杆菌、鲍曼不动杆菌、副溶血弧菌、创伤弧菌和金黄色葡萄球菌;血液中可检出大肠埃希菌和副溶血弧菌;血内毒素含量和白细胞数显著升高。结论动物腹部开放伤合并海水浸泡后可导致多种细菌的混合感染,比单纯腹部开放伤动物感染机会增加,感染程度加重。Objective To investigate the characteristics of bacterial infection in rabbits after the open abdominal wound was immersed with seawater.Methods The animal infection models with open abdominal wounds were built in rabbits and randomly divided into two groups,control group and experimental group.The wounds of rabbits in the experimental group were then immersed into seawater for 20 minutes while those in the control group were not.Bacteria were identified manually and by VITEK Auto Microbe System.Plasma endotoxin content was detected by Microbiology Kinetic Rapid Reader MB-80.White blood cell count was measured by microscope count method.Results Compared with the control group,the amount of bacteria in the wounds of rabbits in the experimental group increased obviously 12h after seawater immersion of the open abdominal wounds.The main bacteria included Escherichia coli,Proteus mirabilis,Acinetobacter baumannii,Vibrio parahaemolyticus,Vibrio vulnificus and Staphylococcus aureus.Escherichia coli and Vibrio parahaemolyticus were also detected in the blood.The plasma endotoxin level and white blood cell count also significantly increased.Conclusions More and severer bacterial infection may develop if the open abdominal wound is immersed with seawater.

关 键 词：腹部开放伤 海水浸泡 细菌 感染 

分 类 号：R332[医药卫生—人体生理学]