RT-LAMP方法在血液HCV筛检中的初步应用  

Application of Reverse Transcription Loop-Mediated Isothermal Amplification Assay in Blood HCV RNA Screening

作　　者：王世华[1] 高菊红 

机构地区：[1]广州军区广州总医院输血科,广东广州510010 [2]广州市越秀区妇幼保健院东山分院

出　　处：《实用预防医学》2012年第6期917-918,共2页Practical Preventive Medicine

摘　　要：目的探讨逆转录环介导等温扩增(reverse transcription loop-mediated isothermal amplification,RT-LAMP)方法在血液丙型肝炎病毒(hepatitis C virus,HCV)筛检中的应用价值。方法利用RT-LAMP法和ELISA法分别对450份血液标本进行HCV检测。结果 RT-LAMP方法检出3份HCV RNA阳性,加荧光染料(SYBR Green I)染色后进行结果判定;ELISA法检出2份抗HCV阳性标本;RT-LAMP法相比ELISA法检出率更高。结论 RT-LAMP法有应用于血液筛检HCV RNA的潜力。Objective To evaluate the application value of reverse transcription loop-mediated isothermal amplification（RT-LAMP） assay in blood hepatitis C virus（HCV） screening.Methods Four hundred and fifty specimens of blood were detected for HCV by RT-LAMP assay and ELISA.Results After RT-LAMP reaction,positive results were observed by SYBR Green I staining in three specimens of blood,while only two positive specimens were detected by ELISA.The detection rate of RT-LAMP assay for HCV RNA is higher than that of ELISA.Conclusions The RT-LAMP assay is a promising approach for blood HCV RNA screening.

关 键 词：丙型肝炎 逆转录环介导等温扩增 血液筛检 

分 类 号：R446.11[医药卫生—诊断学]