银屑病患者皮损和外周血中CD123＋BDCA-2＋浆细胞样树突细胞的表达  

Quantification of CD123＋ BDCA-2＋ plasmacytoid dendritic cells in skin lesions and peripheral blood of patients with psoriasis vulgaris

作　　者：陈静 王豫平[1] 李家文[2] 陈宏翔[2] 李丽娜[1] 李振鲁[1] 

机构地区：[1]河南省人民医院皮肤科,郑州450003 [2]华中科技大学同济医学院附属协和医院皮肤科

出　　处：《中华皮肤科杂志》2012年第7期478-480,共3页Chinese Journal of Dermatology

摘　　要：目的探讨CD123＋BDCA-2＋浆细胞样树突细胞（PDC）在寻常性银屑病患者皮损和外周血中的表达及意义。方法收集22例寻常性银屑病患者和15例健康人皮肤组织和外周血，免疫组化法检测患者皮损和健康人皮肤组织中PDC的表达水平，流式细胞仪检测外周血中PDC的比例。结果免疫组化结果显示，银屑病皮损中PDC的表达水平为（10．1±2．1）／mm2，健康对照组为（0．4±0．6）／mm2，银屑病组显著高于健康对照组（t=17．34，P〈0．01）。银屑病患者外周血中CD123＋BDCA-2’PDC占外周血单一核细胞的比例为（O．17±0．07）％，健康对照组为（0．33±0．20）％，银屑病组显著低于健康对照组（t=4．48，P〈0．01）。结论PDC通过在皮损中重新分布，在银屑病外周血中下降，可能与银屑病的发生、发展相关。Objective To quantify CD123＋ BDCA-2＋ plasmacytoid dendritic cells （PDCs） in skin lesions and peripheral blood of patients with psoriasis vulgaris, and to investigate their significance. Methods Skin tissue samples were reseeted from the lesions of 22 patients with psoriasis vulgaris and normal skin of 15 healthy controls, and peripheral blood samples were also obtained from these subjects. Immunohistochemieal technique （SP） was used to quantify PDCs in skin tissue samples, and flow eytometry to determine the proportion of PDCs in peripheral blood samples. Results Immunohistochemieal study showed that the density of PDCs in the psoriatic lesions was significantly higher than that in the healthy controls （（10.1±2.1）/ram2 vs. （0.4±0.6）/ram2, t = 17.34, P 〈 0.01 ）. On the contrast, the proportion of PDCs in peripheral blood was 0.17% ±0.07% in the patients with psoriasis vulgaris, significantly lower than that in the healthy controls （0.33% ± 0.20%, t = 4.48, P 〈 0.01 ）. Conclusions The proportion of PDCs is reduced in the peripheral blood of patients with psoriasis vulgaris, which may he related to the initiation and progression of psoriasis vulgaris.

关 键 词：银屑病 白细胞介素3受体d亚单位 树突细胞 BDCA-2 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]