132例皮肤疣组织人乳头瘤病毒分型检测  

Detection and genotyping of human papillomavirus in 132 patients with cutaneous warts

作　　者：何泳洁[1] 曹碧兰[1] 汪治国 陈晓红[1] 曾令芬[1] 黄洋[1] 熊琦[1] 颜廷凯[1] 

机构地区：[1]遵义医学院附属医院皮肤科,贵州563000 [2]湖北省咸宁市中心医院同济咸宁医院皮肤科

出　　处：《中华皮肤科杂志》2012年第7期515-516,共2页Chinese Journal of Dermatology

摘　　要：目的探讨皮肤疣组织中人乳头瘤病毒（HPV）的感染率和病毒型别。方法门诊皮肤疣患者132例中寻常疣46例，跖疣38例，扁平疣48例。以特异性DNA序列为模板设计引物，进行PER扩增，电泳，对结果进行分析。结果132例皮肤疣中，HPV阳性128例，感染率为97．0％，其中HPV2型阳性111例，感染率为84．1％。寻常疣、跖疣感染主要为HPV2、HPV1型，扁平疣感染主要为HPV2、HPV10、HPV3、HPV1型。89例为多重感染，感染率为67．4％，其中二重感染率为41．7％（55例），三重感染率为21．2％（28例），四重感染率为4．6％（6例）；寻常疣多重感染率为50．0％（23／46），跖疣为76．3％（29／38），扁平疣77．1％（37／48）。多重感染中，以HPV1＋2型最多，有35例（39．3％），其次为HPV1＋2＋10型，有12例（13．5％）。结论遵义皮肤疣感染以HPV2型为主，其次为1型；2型与多种皮肤疣感染有关；皮肤疣中多重感染率较高，其中扁平疣多重感染率最高；新发现HPV3、10型感染寻常疣，HPV10型感染跖疣，HPV1、7型感染扁平疣。Objective To detect and genotype HPV in cutaneous wart tissue. Methods This study recruited 132 outpatients with cutaneous warts in the Affiliated Hospital of Zunyi Medical College. Of the 132 outpatients, 46 had verruca vulgaris, 38 had verruca plantaris, and 48 had verruea plana. Ten healthy human and 15 patients with condyloma acuminatum served as the control. Tissue specimens were obtained from the lesions of these patients as well as from the normal skin of the healthy controls. DNA was extracted from the specimens followed by PCR amplification with general and type-specific primers. Results Of the 132 wart specimens, 128 （97.0%） were positive for HPV, 111 （84.1%） for HPV2, 89 （67.4%） for multiple types of HPV, 55 （41.7%） for two types of HPV, 28 （21.2%） for three types of HPV, and 6 （4.6%） for four types of HPV. The most prevalent HPV types were HPV2 followed by HPV1 in ven＇uca vulgaris and verruca plantaris, HPV2 followed by HPV10,HPV3 and HPV1 in verruca plana. Multiple types of HPV were identified in 50.0% （23/46） of the verruea vulgaris specimens,76.3% （29/38） of the verruca plantaris specimens,and 77.1% （37/48） of the verruea plana specimens. Among patients infected with more than one type of HPV, the combination of HPV1 and 2 was the most common （35, 39.3%）, followed the combination of HPV1, 2 and 10 （12, 13.5%）. Conclusions The most prevalent HPV type appears to be HPV2, followed by HPV1, in patients with cutaneous warts in Zunyi area of Guizhou province. HPV2 infection is associated with various types of cutaneous warts. There is a high incidence of eoinfection with multiple types of HPV in patients with cutaneous warts, especially in those with verruea plana. Moreover, HPV3 and 10 are newly identified in verruca vulgaris, HPV10 in verruca plantaris,HPV1 and HPV7 in verruea plana.

关 键 词：人乳头瘤病毒 皮肤疣 分型检测 组织 多重感染 感染率 扁平疣 DNA序列 

分 类 号：R7[医药卫生—临床医学]