PCR联合透射电镜诊断口唇初发单纯疱疹一例  

Diagnosis of primary herpes simplex in the upper lip with PCR and transmission electron microscopy： a case report

作　　者：李聪慧[1] 冉玉平[1] 雷松[2] 丁柳[3] 王鹏[1] 

机构地区：[1]四川大学华西医院皮肤科,成都610041 [2]四川大学华西医院病理科,成都610041 [3]四川大学华西医院实验医学科临床分子诊断室,成都610041

出　　处：《中华皮肤科杂志》2012年第7期519-520,共2页Chinese Journal of Dermatology

摘　　要：患者男，66岁，因上唇水疱、糜烂伴疼痛7d就诊。取水疱及痂皮，利用单纯疱疹病毒（HSV）特异性引物做实时荧光PCR检测，发现HSV-1阳性、HSV-2阴性。取疱壁做透射电镜观察，见表皮细胞核内有空心型、颗粒型或致密型3种类型的核衣壳，细胞核外大量成熟病毒颗粒，鉴定为疱疹病毒。诊断：口唇单纯疱疹。PCR分子分型结合透射电镜是确诊初发单纯疱疹的有效方法。A 66-year-old man was admitted for a 7-day history of painful blisters and erosions in the upper lip. Real-time PCR with herpes simplex virus （HSV） type-specific primers showed that the blister fluid and crusts were positive for HSV-1, but negative for HSV-2. Observation of the blister wall with transmission electron microscopy （TEM） revealed 3 types of nucleocapsid in the karyoplast of epithelia cells, including the electron-translucent core, granular core and electron-dense core. Numerous matured viral particles with envelope were found in the cytoplasm, which were identified as HSV. The diagnosis was made as herpes simplex in the upper lip based on the above findings. PCR based molecular typing combined with observation of HSV particles via TEM may be an effective approach to the definite diagnosis of primary herpes simplex.

关 键 词：实时荧光PCR检测 单纯疱疹病毒 透射电镜诊断 口唇单纯疱疹 初发 透射电镜观察 细胞核内 特异性引物 

分 类 号：R752.11[医药卫生—皮肤病学与性病学]