贵州10种玉米基因型组织培养再生体系的建立  

Plant Regeneration using Immature Embryo from Ten Genotypes of Guizhou Maize via Embryogenic Callus

作　　者：田晓娥[1,2] 朱友银[1,3] 赵德刚[1,2,3] 

机构地区：[1]贵州大学贵州省农业生物工程重点实验室,贵州贵阳550025 [2]贵州大学生命科学学院,贵州贵阳550025 [3]贵州大学绿色农药与农业生物工程国家重点实验室培育基地,贵州贵阳550025

出　　处：《山地农业生物学报》2012年第3期194-199,共6页Journal of Mountain Agriculture and Biology

基　　金：贵州省科技厅转基因专项(2004NZ004);国家转基因生物新品种培育重大专项(2008ZX08010-003)

摘　　要：以贵州10种玉米基因型的幼胚为外植体,研究2,4-D浓度和基因型对玉米胚性愈伤组织诱导的影响,并进行再生体系研究。结果表明:不同基因型玉米幼胚诱导胚性愈伤组织的最适2,4-D浓度不同:黔单19、黔单20、黔单23、09H313、大青233、益玉6号母本、益玉6号父本的最适2,4-D浓度为1.0 mg/L,遵玉母本为2.0mg/L,黔单21为3.0 mg/L。另外,不同基因型胚性愈伤组织的诱导能力及分化再生能力差异明显。其中,胚性愈伤组织诱导率较高的是遵玉母本和大青233,分别为81.87%和71.39%,而绿芽分化率较高的是黔单23和黔单21,分别为89.80%和80.00%。贵州10种玉米基因型中黔单23、黔单21和益玉6号父本表现出较好的再生频率,可为农杆菌介导遗传转化提供备选受体。Immature embryos of ten maize （Zea mays L. ） genotypes from Guizhou Province were used to in- duce embryogenic callus. Effects of genotypes and 2,4-D concentrations on the induction of embryogenic cal- lus, as well as the regeneration ability were investigated in present work. The results showed that the optimal 2,4-D concentration for embryogenic callus induction were different depending on the genotypes. Addition of 1.0 mg/L 2,4-D gave the best effect in Qiandanl9, Qiandan20, Qiandan23, 09H313, Daqing233, the par- ent of Yiyu 6 and the male parent of Yiyu6, however the optimal concentrations for the parent of Zunyu and Qiandan21 were 2.0 mg/L and 3.0 mg/L, respectively. Also, induction and differentiation of embryogenic callus diversified among the genotypes. The best induction rate of embryogenic callus were investigated from the parent of Zunyu （81.87%） and Daqing233 （71.39%）, and the highest rate of differentiation were ob- tained from Qiandan23 （89.80%） and Qiandan21 （80.00%）. Among the ten genotypes, Qiandan23, Qi- andan21 and the male parent of Yiyu6 demonstrated the higher regeneration ability, thus may facilitate trans- formation via agrobacterium.

关 键 词：玉米幼胚 胚性愈伤组织 再生体系 2 4-D 基因型 

分 类 号：Q943.1[生物学—植物学]