栽培基质对利用三叶草扩繁AM真菌的影响  

Effects of Culture Substrates on Propagation of Arbuscular Mycorrhizal Fungi Using Trifolium repens L. as Host

作　　者：唐瑶[1] 黄丽萍[1] 江龙[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025

出　　处：《山地农业生物学报》2012年第3期212-216,共5页Journal of Mountain Agriculture and Biology

基　　金：教育部重点项目(2011-167);贵州省社发攻关项目[黔科合SY(2011)3085];贵阳市社发攻关计划项目[筑科合(2011103)51]

摘　　要：采用4×4双因子设计,研究不同栽培基质和AM真菌菌株对三叶草的生长与营养状况及AM真菌侵染的影响。结果表明:基质种类和AM真菌均显著影响三叶草接种AM真菌的侵染率,纯基质中接种BEG-141和BEG-168菌株三叶草的AM真菌侵染率达到90%以上,与沙基质中AM真菌侵染率无显著差异,但纯基质中三叶草的生物量显著大于沙,说明纯基质可以作为AM真菌扩繁的培养基质,这为AM真菌的规模化生产提供了试验依据。Different culture substrates and AM fungal strains on the growth and nutrition of Trifolium repens L. as well as infection of AM fungi were investigated by 4×4 two factor design experiments in the present work. The results showed that different substrates and AM fungi highly influenced the mycorrhizal infection rate. Root colonization rate of Trifolium repens L. , inoculated with BEG-14 and BEG-168 and cultured in pure sub- strate without sand, was higher than 90% , which was not significantly different with those cultured in sand. Biomass of the Trifolium repens L. cultured in pure substrate without sand was significantly elevated in com- parison with that in sand. The obtained evidences suggested that the pure substrate adopted in the experi- ments might be optimal culture substrate for AM fungi propagation, thus can provide an experimental base- ment for enlargement production of AM fungal inoculum.

关 键 词：AM真菌 基质 扩繁 

分 类 号：Q94[生物学—植物学]