农杆菌介导FAD8基因遗传转化小油桐的研究  

Transformation of FAD8 Gene to Jatrapha curcas L. Mediated by Agrobacterium tumefaciens

作　　者：李晨昕[1,2] 李岩[2,3] 赵德刚[1,2,3] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州大学贵州省农业生物工程重点实验室,贵州贵阳550025 [3]贵州大学教育部绿色农药与农业生物工程重点实验室,贵州贵阳550025

出　　处：《山地农业生物学报》2012年第3期217-221,共5页Journal of Mountain Agriculture and Biology

基　　金：贵州小油桐生物柴油产业化关键技术研究与应用项目[黔科合重大专项字(2007)6004];国家转基因新品种培育重大专项--安全转基因技术研究(2008ZX08010-003)

摘　　要：为了创制抗冷性增强的转基因小油桐新种质,本研究以苗龄15 d左右的小油桐无菌苗子叶为外植体,利用农杆菌介导法,将35S启动子驱动能够提高植物不饱和脂肪酸相对含量的拟南芥FAD8基因遗传转化小油桐,通过获得抗性植株,经GUS组织化学染色及PCR检测,证明FAD8基因已整合进入小油桐基因组中。研究获得移栽成活的小油桐转基因株系2个,与对照相比,转FAD8基因植株无明显表型差异。本研究首次获得转FAD8基因小油桐植株,为其抗冷新种质筛选及创制奠定了基础。To improve the cold resistance of Jatrapha curcas L. the cotyledon of the 15-day-old aseptic seed- ling was used as the explant, and FAD8 gene driven by 35S promoter, which may enhance relative content of unsaturated fatty acid in plant, was transformed into Jatrapha curcas L. mediated by Agrobacterium in the current work. The GUS histochemical stainning and PCR detection proved that FAD8 gene were transformed successfully into Jatrapha curcas L. , and two survival plantlets of transgenic J. curcas L. were gained. Com- pared to the wild type, no obvious difference in phenotype was investigated from the transgenic lines. The ob- tained transgenic J. curcas L. with FAD8 gene might lay the foundation for the further creation for cold toler- ance germplasm of this species.

关 键 词：FAD8基因 农杆菌介导 遗传转化 小油桐 

分 类 号：Q943.2[生物学—植物学] S723.132[农业科学—林木遗传育种]