少腹逐瘀丸抗痛经作用机制研究  

Functional Mechanism of Shao Fu Zhu Yu Wan in Resisting Menalgia

作　　者：贺克[1] 刘姣[2] 李清[2] 

机构地区：[1]河北省石家庄市第四医院,石家庄050011 [2]河北医科大学中医学院

出　　处：《河北中医药学报》2012年第2期3-5,51,共3页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：河北省中医药管理局科研计划项目:No 2011004

摘　　要：目的:观察少腹逐瘀丸对缩宫素诱发小鼠痛经的镇痛作用及作用机制。方法:取KM小鼠50只,随机分为空白对照组、模型组、丹莪妇康煎膏组、少腹逐瘀丸高剂量组及低剂量组。除空白对照组外,其余各组小鼠灌胃给药同时灌服乙烯雌酚,连续7天。末次给药30 min后,除空白对照组外,其余各组小鼠腹腔注射缩宫素,观察小鼠离体子宫平滑肌收缩变化,小鼠30 min内扭体反应次数,并检测小鼠血浆血栓烷B2(TXB2)、6-酮-前列腺素F1α(6-Keto-PGF1α)及血清超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果:少腹逐瘀丸能够明显抑制缩宫素诱导小鼠离体子宫平滑肌收缩(P<0.05),使痛经小鼠扭体反应次数有降低趋势,但差异无显著性(P>0.05),并能明显降低小鼠血浆TXB2含量(P<0.05),明显升高血浆6-Keto-PGF1α含量(P<0.05),显著降低血清MDA含量(P<0.01),明显升高血清SOD含量(P<0.05)。结论:少腹逐瘀丸对缩宫素诱导小鼠痛经模型具有较好治疗作用,其作用可能与调节TXB2/6-Keto-PGF1α比值、抗氧化有关。Objective:to observe the analgesic effect and the functional mechanism of Shao Fu Zhu Yu Wan on oxytocin-induced menalgia of mice. Method: 50 KM mice were randomly divided into control group, model group, soft extract group of Dan'e Fukang, high dose and low dose groups of Shao Fu Zhu Yu Wan, all of which, except the first group, were given intragastric administration and diethylstilbestrol for 7 days successively, then intraperitoneal injection with oxytocin 30 minutes after the last administration, for the purpose to observe the contraction changes of the uterus smooth muscle in vitro, frequency of writhing response in 30 minutes and the following contents: TXB2, 6-Keto-PGF1, SOD and MDA. Results: Shao Fu Zhu Yu Wan could inhibit significantly the muscle contraction of the uterus in vitro induced by oxytocin (P<0.05), lower the frequency of the writhing response without significant difference (P>0.05), significantly decrease TXB2 (P<0.05), increase 6-Keto-PGF1 (P<0.05), decrease MDA (P<0.01) and increase SOD (P<0.05). Conclusion: Shao Fu Zhu Yu Wan has a better therapeutic effect on mice with oxytocin-induced menalgia, and the functional mechanism may be relevant to the regulation of TXB2/6-Keto-PGF1 ratio and the anti-oxidant.

关 键 词：少腹逐瘀丸 痛经 血栓烷B2 6-酮-前列腺素F1Α 超氧化物歧化酶 丙二醛 

分 类 号：R285.5[医药卫生—中药学]