响应面法优化嗜盐紫色硫细菌283-1培养基  

Optimization of Medium Composition of the Halophilic Purple Sulfur Bacterium 283-1 Using the Response Surface Methodology

作　　者：崔小华[1] 杨素萍[2] 姚鹏[3] 

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]华侨大学生物工程与技术系,福建厦门361021 [3]山西中医学院中西医结合临床医学系,山西太原030024

出　　处：《山西农业大学学报（自然科学版）》2012年第1期37-41,共5页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(31070054);福建省自然科学基金(2010J01209)

摘　　要：为了提高紫色硫细菌283-1的生物量,对其培养基组分进行优化。采用单因子试验、Plackett-Burman设计法、最陡爬坡试验及中心组合设计响应面分析,研究了乙酸钠、碳酸氢钠、氯化铵、硫化钠、硫酸镁和无机盐6个因子对菌株283-1生物量的影响。结果表明,乙酸钠和硫化钠是影响该菌株生物量的主要因素,并获得了最佳培养基配方:乙酸钠1.45g·L-1,碳酸氢钠0.75g·L-1,氯化铵4.5g·L-1,硫酸镁0.5g·L-1,硫化钠1.05g·L-1,氯化钙0.19g·L-1,氯化钾0.34g·L-1,氯化钠50g·L-1,VB1220μg·L-1,无机盐溶液1mL·L-1。优化后菌株283-1的生物量提高了6倍,结果与理论预测值相近。In order to improve the biomass,medium composition of the strain 283-1 of purple sulfur Bacterium was opti mized. Effects of the six factors CH：3COONa, NaHCO3, NH3Cl, Na2S, MgSO, and inorganic salt on the biomass were evaluated by using single-factor test, Plackett-Burman design,the steepest ascent experiment and central compos- ite design combined with response surface analysis in this work. The results showed that sodium acetate and sodium sul- fide were the most significant factors and the optimal media were as following： CH：3COONa 1.45 g·L-1 , NaHCO3 0.75 g·L-1 , NH,C1 4.5 g·L-1 , MgSO4 0.5 g·L-1 , Na.,S 1.05 g·L-1 , CaCl2 g·L-1 , KC1 0.34 g·L-1 , NaCI 50 g·L-1, V1312 20 g·L-1 and inorganic salt 1 mL·L-1. With the optimized media, the biomass improved 6 times compared with the control and the value was in agreetment with the predicted.

关 键 词：紫色硫细菌 生物量 响应面分析 培养基组分 

分 类 号：Q93-335[生物学—微生物学]