利用AhMITE1转座子分子标记鉴定花生F_1代杂种  

Identification of Peanut F_1 Hybrid Using AhMITE1 Transposable Element Marker

作　　者：王洁[1,2] 李双铃[2] 王辉[2] 石延茂[2] 任艳[2] 何国浩 袁美[1,2] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]山东省花生研究所,山东青岛266100 [3]Department of Agricultural Sciences,College of Agricultural,Environmental and Natural Sciences,Tuskegee University,Tuskegee,AL36088,USA

出　　处：《花生学报》2012年第2期8-12,共5页Journal of Peanut Science

基　　金：山东省博士基金项目(BS2011NY014);作物种业科技工程项目(2011BAD35B04);留学人员科技活动择优资助项目;国家自然科学基金项目(30700509);科技部“863”项目(2006AA100106)

摘　　要：对杂交后代进行杂种的真实性鉴定是杂交育种成功的前提。AhMITE1转座子标记是一类基于PCR的分子标记,其在花生中扩增的产物片段差异大,多态性强,采用琼脂糖凝胶电泳即可区分。本研究用7对多态性转座子引物对6个花生组合的166个F1单株进行了真实性鉴定。根据杂种植株含有父母本带型为依据共鉴定出了77个单株为真杂种,真杂种植株数占植株总数的比率为44.86%;收获时根据田间表型鉴定出了79个单株为真杂种,真杂种比率为47.59%。Accurate identification of F1 hybrid is critical to hybridization breeding in peanut. MITE transposable marker is a type.of PCR marker with high polymorphism and easy detection by agarose gel electrophoresis. 7 pairs of polymorphic primers were used to amplify 166 F1 individual plants from 6 crosses. Out of those plants, 77 plants were identified as true hybrids, accounting for 44,86%. Based on phenotype and agronomic traits, 79 plants were identified as true hybrids, accounting for 47.59%.

关 键 词：花生 杂种鉴定 分子标记 转座子 AhMITE1 PCR 

分 类 号：S565.2[农业科学—作物学] Q963[生物学—昆虫学]