HIV-1毒株gag和pol基因区抗原表位及耐药性突变分析  

Analyses on Antigen Epitopes and Drug Resistance Mutations of HIV-1 gag and pol Genes

作　　者：尚诚彰[1,2] 陈国敏[1] 张怀渝[2] 曾毅[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052 [2]四川农业大学生命科学与理学院,四川雅安625014

出　　处：《病毒学报》2012年第4期351-357,共7页Chinese Journal of Virology

基　　金：973计划(2005CB522903和2006CB04207)

摘　　要：通过研究HIV-1 gag和pol基因序列变异特征,以了解抗原表位变异特点和耐药性突变情况。采集HIV-1阳性全血样本,使用巢式PCR扩增gag和pol基因部分区段,得到23份样本的PCR序列和相应样本的克隆序列gag基因为449份、pol基因为402份,基因分型为HIV-1B和B′亚型。两种亚型的共享序列在选取的8个CTL抗原表位中,共有4个抗原表位发生了8个突变位点;另外位于p24区的5个抗原表位在PCR序列中没有发生变化,但在它们的克隆序列(9.80%)中发生了少数突变。在pol基因PCR序列和克隆序列中发现蛋白酶抑制剂(Protease inhibitors,PIs)和逆转录酶抑制剂(Reverse transcriptase inhibitors,RTIs)耐药性突变位点分别为18个和24个,其中只在克隆序列中发现的PIs和RTIs耐药性突变位点分别占其总数的94.44%(17/18)和62.50%(15/24)。结果显示本研究样本PCR序列中表现出耐药性的比例较高(17.39%),并在克隆序列中存在少数免疫逃逸和耐药性突变,提示部分样本对现行抗病毒药物产生一定程度的耐药。To study the CTL antigen epitopes and drug resistance mutations of HIV-1 gag and pol genes through analyzing gag and pol gene sequences. The HIV-1 gag and pol gene fragments were amplified using nested polymerase chain reaction. A total of 23 PCR sequences, 449 cloned gag sequences and 402 cloned pol sequences were obtained. Sequence analyses showed the 23 samples were subtype B or Bt. A to- tal of 4 in 8 CTL antigen epitopes appeared 8 mutations in consensus sequence of subtype B and Br. There were no mutations found in the PCR sequences, whereas a few mutations were found in clone sequences （9.80%）in 5 antigen epitopes in p24 region. Eighteen PIs-related mutations and 24 RTIs-related mutations were found in PCR sequences and clone sequences in pol gene region, in which 17 （94.44%） PIs-related mutations and 15 （62.50%） RTIs-related mutations were found only in the clone sequences, respectively. The results showed that the prevalence of HIV-1 drug resistance strains in this study was at a higher level （17.39%）, suggesting that some samples were resistant to existing antiviral drugs.

关 键 词：HIV-1 GAG POL 抗原表位 耐药性突变 

分 类 号：R373.9[医药卫生—病原生物学]