中国HIV-1 B′亚型毒株不同基因区与全长基因组系统进化比较  

Comparison between Different Gene and Near Full-length Genome on the Phylogenetic Analyses of HIV-1 Subtype B′ in China

作　　者：李喆[1,2] 杨尧[1] 魏静[1] 冯毅[1] 邢辉[1] 何翔[1] 邵一鸣 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制国家重点实验室,北京102206 [2]南开大学,天津300071

出　　处：《病毒学报》2012年第4期366-371,共6页Chinese Journal of Virology

基　　金：“十一五”科技重大专项(2008ZX10001-004);863计划(2006AA022418);国际科技合作计划(2009DFB30420)

摘　　要：本研究旨在探索不同基因区的使用对HIV-1B′亚型毒株系统进化分析结果的影响。首先利用既往研究中已发表的共计47条来自泰国,缅甸和中国多个地区不同传播途径的B′毒株近全长基因组序列,将其按基因区分为不同的数据集(gag,pol,vif,vpr,vpu,env,nef),并分别进行系统进化分析研究。比较不同基因区系统进化分析的结果发现,B′亚型毒株pol基因在分析的基因区中,具有最低的复杂度和进化速率,可以较好的区分B′TH和B′YN毒株,重复近全长基因组序列的分析结果;尽管env基因则具有最高的复杂度和进化速率,但无法获得类似结果。本研究比较了不同基因区对HIV-1B′亚型毒株系统进化分析结果的影响,对进一步开展HIV分子流行病学调查,分析我国B′毒株在我国的传播奠定了基础。This study aims to compare the influence of different genes to the results of HIV-1 subtype B′ phylogenetic analyses. We first split 47 near full-length genome sequences of subtype B′ into different regions （gag, pol, vif, vpr, vpu, env, he f）, which derived from various risk populations and geographic regions from Thailand, Myanmar and China from published studies. Phylogenetic analyses were performed to each region obtained. The phylogenetie results of different regions were compared to that of the near full-length genome sequences. The pol gene was found to have the lowest diversity and evolutionary rate, and could repeat the phylogenetic results by using near full-length genome sequences. Although the env gene has the highest diversity and evolutionary rate, it could not achieve the similar results. This study compared the influence to the results of HIV-1 subtype B′ phylogenetic analyses by using different genes and laid foundation for further molecular survey and analyses of the transmission of subtype B′ in China.

关 键 词：HIV-1 B'亚型 pol基因 近全长基因组 

分 类 号：R373.9[医药卫生—病原生物学]