猪博卡病毒环状附加体的发现与鉴定  

Identification and Characterization of Porcine Bocavirus Episomes

作　　者：杨晚竹[1] 黄灿平[1] 段招军[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052

出　　处：《病毒学报》2012年第4期418-423,共6页Chinese Journal of Virology

基　　金：传染病防治重大专项(2009ZX10004-101)

摘　　要：为了证实博卡病毒可以环状附加体形式存在于宿主体内,本研究利用半巢式PCR方法在健康猪粪便标本中筛查出2株猪博卡病毒环状附加体PBoV_G2-episome和PBoV_G3-episome。通过反复测序和序列拼接得到其末端非编码区序列(405nt和511nt)。经过对其进行序列分析及二级结构的预测,发现PBoV_G2-episome与人博卡病毒3附加体(HBoV3-episome)结构相似,而PBoV_G3-episome与博卡病毒属其他成员的末端二级结构存在较大差别。猪博卡病毒环状附加体的发现证实了有些博卡病毒与其他细小病毒的复制方式存在一定差异,也为今后博卡病毒感染性克隆的构建提供了一条研究思路。To verify that the circular forms of bocavirus genome exist in their host, bocavirus episomes were detected in fecal samples of healthy piglets using a semi-nested PCR method. Two species of porcine bocaviruses （PBoVG2-episome and PBoVG3-episome） were identified for the first time. The relevant terminal sequences of the noncoding region （405 and 511 nt, respectively） were also obtained. Sequence analyses and secondary structure prediction indicated that the PBoVG2-episome was more similar to that of human boeavirus 3 （HBoV3） but the PBoVG3-episome was quite different from that of other members of the genus Bocavirus. Discovery of episomal forms of porcine bocaviruses （PBoV） suggested that PBoV, like HBoV, used a different replication mechanism from other parvoviruses. The sequencing of episome Inverted Terminal Repeats （ITRs） also contributes to a possible alternative strategy for constructing infectious molecular clones of bocavirus in a future study.

关 键 词：博卡病毒 附加体 半巢式PCR 

分 类 号：S852.659.2[农业科学—基础兽医学]