黏质沙雷菌短尾噬菌体中国分离株的鉴定  

Characterization of a Novel Podoviridae-phage Infecting Serratia Marcescens Isolated in China

作　　者：徐凤宇[1,2] 刘永杰[3] 马红霞[1,2] 张岩[1,2] 苏胜兵[1,2] 沈婵娟[3] 陆承平[3] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118 [2]吉林农业大学动物生产及产品质量安全教育部重点实验室,吉林长春130118 [3]南京农业大学动物医学院,江苏南京210095

出　　处：《病毒学报》2012年第4期439-443,共5页Chinese Journal of Virology

基　　金：吉林省自然科学基金项目(201115191)

摘　　要：以黏质沙雷菌jn01株为宿主菌,从环境污水中分离噬菌体,经反复挑取噬菌斑,获得1株纯化的噬菌体,定名为SmPjn。SmPjn在双层琼脂平板上可形成直径约2mm,圆形、透明的噬菌斑,边缘清晰。透射电镜观察,该噬菌体有一短尾,长(7±1.25)nm,头部长、宽分别为(58±2.16)nm×(55±0.47)nm,属短尾噬菌体科(Podoviridae)。可裂解jn01以外的2株黏质沙雷菌;与宿主菌共培养4h后的最佳感染复数为1;一步生长曲线表明该噬菌体的潜伏期约为50min,平均爆发量约为1125pfu/cell。基因组为大于27kb的DNA,可分别被HindⅢ、EcoRⅠ切成11和9个电泳片段。本报道为国内首次分离黏质沙雷菌短尾噬菌体。Serratia marcescens jn01 was employed as the host for the isolation ot phages trom environmental sewage. One strain of phage named SmPjn was purified by picking transparent plaque with 2mm diameter and clear edge on the double - layer agar repeatedly. Electron micrographs indicated that the phage head was ieosahedral with head size and tail length of （58±2.16）× （55±0.47）nm and （7±1.25）nm, respectively. On the basis of the morphology, this phage belongs to the family Podoviridae. Host-range determination revealed that the phage was capable of infecting the other two isolates of S. marcescens, P25 and CMCC41002. The optimal multiplicity of infection was 1. A one-step growth curve of SmPjn indicated that the latent period and burst size were estimated at 50min and 1,125 pfu/cell, respectively . Genomic DNA of SmPjn was above 27kb in size and could be digested by Hind III and EcoR I into 11 and 9 visible fragments after electrophoresis, respectively. A novel Podoviridae-phage infecting S. marcescens was firstly reported in China.

关 键 词：黏质沙雷菌 短尾噬菌体 分离 鉴定 

分 类 号：R378.2[医药卫生—病原生物学]