hVEGF165/Ang-1联合转染对兔缺血心肌血管再生的影响  

The effect of combination of hVEGF165/Ang - 1 gene transfection on angiogenesis in rabbit ischemic myocardium

作　　者：陈李李[1] 袁延亮[1] 董红燕[2] 张中明[1] 张昊[1] 王正[1] 

机构地区：[1]徐州医学院附属医院胸心外科,江苏徐州221002 [2]徐州医学院神经生物学研究中心,江苏徐州221002

出　　处：《徐州医学院学报》2012年第5期286-289,共4页Acta Academiae Medicinae Xuzhou

摘　　要：目的 探讨VEGF/Ang-1基因联合转染对缺血心肌区域血管再生及血管成熟的影响.方法 建立兔心肌缺血模型,实验动物随机分为5组（每组5只）：hVEGF165/Ang-1双基因联合转染组（A组）,hVEGF165单基因转染组（B组）,Ang-1单基因转染组（C组）,单纯梗死组（D组）,正常对照组（E组）.冠状动脉左前降支（LAD）结扎后在缺血心肌区域直接注射的方法转染 rAAV-9HRE-hVEGF165和（或）rAAV-TRE-Tight-Ang-1;Fitc-lectin和α-SMA免疫荧光染色,观察缺血心肌区域血管新生及成熟情况.结果 与B、C、D组相比,A组Fitc-lectin和α-SMA阳性血管数量显著增多（P〈0.05）.结论 与单一促血管生成基因转染比较,hVEGF165/Ang-1双基因联合转染可改善兔心肌梗死区域血管再生.Objective To test the effect of combination of vascular endothelial growth factor （VEGF） and angiopoietin- 1 （Ang- 1 ）gene transfection on angiogenesis and vascular maturation in ischemic myocardium. Methods The rabbit model of myocardial isehemia was established by ligating the left ventricular anterior descending coronary artery branch. The experimental animals were ramdomly divided into 5 groups （n = 5 each） ：hVEGF165/Ang- 1 doublegene group （group A）, hVEGFt65 single -gene group （group B）, Ang- 1 singlegene group （group C）, infarct group （group D） , normal control group （group E）. Target genes were transferred to ischemie myocardium by direct intramuscular injection. Angiogenesis and vascular maturation during ischemic myocardium were observed in each group using immunofluorescence staining. Results Fitclectin and α -SMA positive vessels were significantly increased in group A compared to groups B, C and D （ P 〈 0.05 ）. Conclusion Compared to single gene transfection, combination of VEGF and Ang - 1 gene transfection can enhance the angiogenesis in ischemic area of myocardium in rabbits.

关 键 词：心肌缺血 血管内皮生长因子 血管紧张素-1 基因治疗 血管再生 

分 类 号：R542.2[医药卫生—心血管疾病]