高效液相色谱法测定白消安血药浓度  

Determination of busulfan in human plasma by HPLC

作　　者：王来成[1] 云筠筠[1] 李振宇[2] 董超[1] 韩强[1] 吕冬梅[1] 邵珠民[1] 

机构地区：[1]徐州医学院附属医院药剂科,江苏徐州221002 [2]徐州医学院附属医院血液科,江苏徐州221002

出　　处：《徐州医学院学报》2012年第5期337-340,共4页Acta Academiae Medicinae Xuzhou

基　　金：基金项目：江苏省卫生厅奥赛康临床药学研究基金项目（P200963）

摘　　要：目的 建立测定人血浆中白消安浓度的柱前衍生高效液相色谱法.方法 以1,5-戊二醇二甲磺酸酯为内标,血浆样品经二乙基二硫代氨基甲酸钠衍生化处理后,以乙腈-0.7%冰醋酸水溶液（69：31）为流动相,流速1.0 ml·min^-1,采用XterraRP18色谱柱分离,在280 nm波长下进行检测.结果 白消安线性范围为0.20～4.00 mg·L^-1,以加权最小二乘法计算得到回归方程为y=2.56x＋16.7（r=0.999 3,n=7）,提取回收率81.01%～82.72%,方法回收率96.50%～97.90%,日内、日间精密度（RSD）均小于10%.最低血浆检测质量浓度为0.080 mg·L^-1.结论 该方法准确、灵敏,适用于白消安血浆浓度测定及其药代动力学研究.Objective To establish a method for determination of plasma busulfan concentration by pre - column derivatization pre - high performance liquid chromatography. Methods 1,5 - diol dimethyl sulfonate was used as internal standard, plasma samples were processed by derivatization with acetonitrile -0.7% acetic acid （69：31） as mobile phase, at a flow rate of 1 ml · min^-1 , Xterra RP18 column （5 μ1m, 4.6 mm ×250 mm ） was used, at 280 nm wave- length detection. Results Busulfan plasma concentration of the linear range of 0. 20 - 4.00 mg ·L ^-1, computing the weighted least squares regression equation for the y = 2. 56x ＋ 16. 7 （ r = 0. 999 3, n = 7 ）. The extraction recovery was 81.01% -82.72% and the method recovery was 96.50% -97.90%. The intra -and inter -day RSD were less than 10%. Minimum plasma concentration of detection was 0. 080 mg· L^-1. Conclusion This method is accurate, sensitive and suitable for determination of busulfan plasma concentration and pharmacokinetic studies.

关 键 词：高效液相色谱法 白消安 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R979.1[医药卫生—药学]