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作 者:朱曼丽[1] 焦谊[2] 王烨[1] 毛新民[3] 李琳琳[1]
机构地区:[1]新疆医科大学基础医学院药理学教研室,乌鲁木齐830011 [2]新疆医科大学基础医学院生物化学与分子生物学教研室,乌鲁木齐830011 [3]新疆医科大学中医学院,乌鲁木齐830011
出 处:《新疆医科大学学报》2012年第6期711-715,共5页Journal of Xinjiang Medical University
基 金:国家自然科学基金(81060068)
摘 要:目的比较新鲜与陈旧外周抗凝血中miRNA(microRNA)表达的变化。方法取5份新鲜及6份陈旧外周抗凝血液样本,提取全血总RNA并测定其纯度与浓度,应用实时定量RT-PCR技术分析外周血中miRNA的表达变化。结果经过核酸蛋白定量仪检测,新鲜与陈旧外周抗凝血液中总RNA A260/280值为1.8~2.0范围内。实时荧光定量RT-PCR的结果显示:新鲜与陈旧的外周抗凝血液中miRNA RNAU6-2的相对拷贝数差异无统计学意义。结论新鲜与陈旧外周抗凝血液中miRNA RNAU6-2均有表达且无差异。Objective To compare the expression of miRNA(microRNA) between fresh and old peripheral anticoagulant blood.Methods 5 pieces of fresh blood samples and 6 pieces of old blood samples were chose respectively,extracted total RNA from the whole blood and measure its purity and concentration,and analyzed the microRNA expression using real-time quantitative reverse transcription polymerase chain reaction(real-time RT-PCR).Results With Micro-spectrophotometer test,A260/280 of total RNA was 1.8~2.0.The comparative number of copies of miRNA RNU6B-2 has no statistical difference between fresh and old peripheral anticoagulant blood.Conclusion There was no difference between miRNA RNU6B-2 expressed in fresh and old peripheral anticoagulant blood samples.
关 键 词:外周抗凝血 微小RNA 实时定量逆转录聚合酶链反应
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