阿托伐他汀抑制ox-LDL诱导的人脐静脉内皮细胞表达胸腺基质淋巴细胞生成素TSLP的表达  

Atorvastatin inhibited the expression of thymic stromal lymphopoietin in cultured vascular endothelial cell induced by ox-LDL

作　　者：昌薇[1,2] 何少林[1] 林静[1] 赵卉[1] 李大主[1] 

机构地区：[1]华中科技大学同济医学院协和医院心内科,武汉430022 [2]武汉市第一医院心内科,武汉430022

出　　处：《中国免疫学杂志》2012年第6期489-492,共4页Chinese Journal of Immunology

基　　金：国家自然基金资助项目(No.81170258)

摘　　要：目的:观察阿托伐他汀对ox-LDL诱导人脐静脉内皮细胞(HUVECs)表达胸腺基质淋巴细胞生成素(TSLP)的影响。方法:将HUVECs分为3组,对照组加50 mg/L的ox-LDL培养12小时;实验组分为2组,分别加50 mg/L的ox-LDL和不同浓度的阿托伐他汀培养12小时,于6小时和12小时进行检测,用ELISA法检测细胞培养上清液中TSLP的浓度,用RT-PCR检测TSLP mRNA的表达,采用免疫荧光检测细胞胞浆中TSLP表达。结果:ELISA和IF结果显示,实验组上清液和细胞浆内的TSLP的表达明显低于对照组,并随浓度的增大及时间的延长,TSLP降低得更明显。结论:阿托伐他汀可抑制ox-LDL诱导的HUVECs表达TSLP,这可能是阿托伐他汀抗动脉粥样硬化炎症反应的机制之一。Objective：To explore effect of atorvastation on expression of thymic stromal lymphopoietin（TSLP） in primary HUVEC induced by ox-LDL.Methods： HUVECs mixed with ox-LDL（50 mg/L） for 12 hours were divided into three groups： the control group was treated with PBS;A1 group was added atorvastatin（1 μmol/L）;A2 group was added atorvastatin（10 μmol/L）.At 6 h and 12 h,TSLP protein in supernatant and cells was assessed by ELISA and immunofluorescence（IF）,TSLP mRNA in HUVECs was detected by RT-PCR. Results：The control group expressed high TSLP.Incubation of HUVEC with atorvastatin resulted in a significant decrease of TSLP mRNA and protein release from HUVEC,which was in a dose-dependent manner.The same results were showed about TSLP protein in supernatant and cells.Conclusion：Atorvastatin can inhibited the expression of TSLP in cultured vascular endothelial cells induced by ox-LDL,which may be one of the mechanisms that statins inhibit inflammation in atherosclerosis.

关 键 词：阿托伐他汀 胸腺基质淋巴细胞生成素 氧化低密度脂蛋白 血管内皮细胞 

分 类 号：R541[医药卫生—心血管疾病]