IL-2协同抗CD45RB抗体调节Treg/Th17细胞的比例并延长小鼠移植皮肤存活时间  

IL-2 synergy CD45RB mAbs regulate the ratio of Treg/Th17 cells and prolong the survival times of the skin allografts in mice

作　　者：郭伟坚 简优强 张国超 邓春艳 齐晖 李富荣 周汉新 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院临床研究中心,深圳518020

出　　处：《中国免疫学杂志》2012年第6期525-529,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.30772042)资助

摘　　要：目的:研究IL-2在抗CD45RB抗体诱导免疫耐受中对Treg/Th17细胞分化的影响,进一步阐明抗CD45RB抗体诱导免疫耐受的机制。方法:用免疫磁珠分选C57BL/6小鼠脾脏中的CD4+T细胞,在抗CD45RB抗体与IL-2的作用下培养72小时后,流式检测Treg/Th17细胞的变化。以BALB/c小鼠为供体,C57BL/6小鼠为受体建立同种异基因皮肤移植模型,分别给予抗CD45RB抗体及IL-2等治疗,术后1、3、5、7、9天取受体鼠脾细胞,动态检测Treg/Th17细胞的变化;术后第9天取移植皮肤HE染色观察炎性细胞的浸润情况;观察并记录移植皮肤的存活时间。结果:CD4+T细胞在IL-2联合抗CD45RB抗体的作用下培养72小时后,Treg比例升高,Th17细胞比例下降;IL-2联合抗CD45RB抗体治疗后明显延长小鼠移植皮肤的存活时间。结论:IL-2可以明显增强抗CD45RB抗体诱导免疫耐受的形成,使Treg细胞上调,下调Th17细胞,有利于免疫耐受的形成。Objective：To investigate the role of IL-2 on the differentiation of Treg/Th17 cells in anti-CD45RB mAbs inducing immune tolerance with anti-CD45RB mAbs,and elaborate the mechanism of tolerance induction by anti-CD45RB mAbs further.Methods：CD4＋ T cells were harvested from the spleen of C57BL/6 mice by immune magnetic beads and cultured in the present of anti-CD45RB mAbs and IL-2 for 72 h and the ratio of Treg/Th17 cells were analyzed by flow cytometry.In vivo,BALB/c mice as donor,C57BL/6 mice as recipient to establish skin transplantation model,recipients were received anti-CD45RB mAbs and IL-2 treatment.Splenocytes were harvested on postoperative 1,3,5,7,9 days for dynamic testing Treg/Th17 cells.On postoperative day 9,the skin allografts infiltrated by inflammatory cells were observed by HE stain;skin grafts survival time were observed and recorded for survival studies.Results：CD4＋T cells cultrued with IL-2 in the present of anti-CD45RB mAbs for 72 h,the ratio of Treg was enhanced and Th17 was reduced;mice combined treatment of IL-2 and anti-CD45RB mAbs can remarkably prolonged the survival time of skin grafts.Conclusion：IL-2 can significantly enhance anti-CD45RB mAbs induce immune tolerance through up-regulate Treg cells and down-regulate Th17 cells.

关 键 词：免疫耐受 CD45RB IL-2 TREG细胞 TH17细胞 

分 类 号：R392.4[医药卫生—免疫学]