γ-干扰素在升主动脉瘤和DebakeyⅠ型胸主动脉夹层中的表达研究  

Expression of interferon-gamma in type Ⅰ thoracic aortic dissections and ascending thoracic aortic aneurysms

作　　者：张雷[1] 廖明芳[1] 田磊[1] 裴轶飞[1] 柳漪薇[1] 沈恬[1] 景在平[1] 

机构地区：[1]上海第二军医大学附属长海医院血管外科,上海200433

出　　处：《中国现代普通外科进展》2012年第6期447-450,共4页Chinese Journal of Current Advances in General Surgery

基　　金：国家自然科学基金(30600599);上海市领军人才基金(LJ06025)

摘　　要：目的:探讨γ-干扰素在Debakey Ⅰ型胸主动脉夹层(TAD)和升主动脉瘤(ATAA)中的表达情况及可能作用机制。方法:TUNEL染色、免疫组织化学染色、western blot、实时定量PCR分别检测11例TAD,10例ATAA及7例对照主动脉的中膜细胞凋亡、MMP-9、INF-γ、p38和J NK蛋白在主动脉中膜中的表达和定位,并分析INF-γ的表达与中膜平滑肌细胞凋亡、MMP-9、p38和JNK蛋白表达的关系。结果:TAD和ATAA组中膜细胞凋亡较对照组高(P<0.01)。IFN-γ和MMP-9在TAD和ATAA组中的表达较对照组明显升高(P<0.01),而两组间无明显差异。TAA组中,IFN-γ与MMP-9、JNK和中膜细胞凋亡呈正相关(P=0.02,0.02,P<0.01)。结论:INF-γ在升主动脉瘤中高表达,可能通过上调MMP-9的表达和/或增加中膜细胞凋亡起作用,作用的信号传导通路可能是J NK信号转导通路。Objective： To examine expression of interferon- γ and their possible roles in aortic dissections and aneurysms. Methods： Aortic specimens were obtained from patients with type I thoracic aortic dissection, ascending thoracic aortic aneurysms, and control organ donors. The expression of interferon-γ, matrix metalloproteinase-9, and signal transduction factors phospho-p38 and phospho-JNK were detected by real time RT-PCR, western blot, and immunohistochemistry, respectively. TUNEL staining was performed to detect apoptosis of media cells. The correlation of these factors and apoptosis was also studied. Results： Apoptosis in the media of thoracic aortic dissection and ascending thoracic aortic aneurysms was dramatically higher than control group. Expression of interferon-γ and matrix metalloproteinase-9 was significantly increased in the media of thoracic aortic dissection and ascending thoracic aortic aneurysms compared with control group（P〈0. 01, respectively）. There were positive correlations between interferon-γ Vs matrix metalloproteinase-9, interferon-γ Vs phospho-JNK, interferon-γ Vs apoptosis in ascending thoracic aortic aneurysms （P=0.02, 0.02, P〈0.01）. Conclusions： Interferon- y might effect the formation of ascending thoracic aortic aneurysms possibly through the up-regulation of matrix metalloproteinase-9 and apoptosis of media cells in humans.

关 键 词：动脉瘤 胸主动脉 γ-干扰素·蛋白酶 

分 类 号：R654.3[医药卫生—外科学]