不同途径肝细胞移植对肝硬化大鼠模型肝脏血流灌注的影响  

Influence of Hepatocyte Transplantation by Different Pathways on Liver Perfusion of Rats with Cirrhosis

作　　者：向贤宏[1] 陈伟[1] 陈柳琴[2] 庄文权[1] 关键[1] 杨建勇[1] 

机构地区：[1]中山大学附属第一医院医学影像科,广东广州510080 [2]广东省人民医院检验科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2012年第3期357-360,367,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金(S2011040002502);中山大学学生业余科研项目(2011)资助

摘　　要：【目的】采用CT灌注成像的方法评价不同途径移植肝细胞对肝硬化大鼠模型肝脏血流灌注的影响,进而筛选出针对肝硬化大鼠模型安全、有效的细胞移植途径。【方法】50只经证实成模的肝硬化大鼠模型随机分为5组,前4组分别自肝动脉、门静脉、脾动脉及尾静脉移植肝细胞悬液2 mL(细胞数量2×106),对照组不干预。各组大鼠在细胞移植前1周及移植后第4、8周行CT灌注扫描,计算肝动脉灌注量(HAP)、门静脉灌注量(PVP)、总肝灌注量(THBP)等参数,死亡大鼠或第8周后处死大鼠取肝脏、肺行病理切片检查。【结果】移植后4周,G1组、G2组与G3、G4、G5组间HAP水平有显著性差异(P<0.05);移植后第8周5组间HAP没有显著性差异(P>0.05)。移植后4、8周,G1组、G2组与G3、G4、G5组间PVP水平差异没有统计学意义(P>0.05)。5组大鼠肝纤维化均未见明显改善,肝假小叶未见明显改建。【结论】不同途径肝细胞移植虽不能有效地改善肝脏的血流灌注,但肝动脉、门静脉及尾静脉途径移植大鼠有较高的病死率,而经脾动脉途径移植相对较为安全。[Objective ]To evaluate the influence of hepatocyte transplantation by different pathways on liver perfusion of rats with cirrhosis using CT perfusion imaging scans to select safe, reliable and effective means of transplantation. [ Methods ] Fifty rats of the hepatic cirrhosis model were randomly divided into 5 groups. The first g groups （G1, G2, G3, and G4） were transplanted 2 mL epatocytes suspension （cell number of 2 × 10^6） through intra-hepatic artery （G1）, portal vein （G2）, splenic artery （G3）, and tail vein （G4）, respectively, The control group （G5） were not intervened with at all. Liver CT perfusion imaging scans were performed in the rats of each group before 1 week and 4, 8 weeks after transplantation. The amount of hepatic artery perfusion （HAP）, portal vein perfusion （PVP）, total hepatic perfusion （THBP） was calculated and analyzed statistically. [ Results ] Four weeks after transplantation, the differences of HAP levels in G1 group, G2 group versus G3, G4, G5 groups were statistically significant （P 〈0.05）. Eight weeks after transplantation, the differences of HAP levels among 5 groups were not statistically significant （P 〉 0.05）. Four and 8 weeks after transplantation, there was no statistically significance in the variation of PVP levels in G1 group, G2 group versus G3, G4, G5 difference （P 〉 0.05）. [ Conclusion ] The results show that hepatocytes transplantation by different pathways can not be effective in improving liver perfusion. On the contrary, liver transplantation by hepatic artery and portal vein increased the risk of disorders.

关 键 词：肝细胞 移植 CT灌注 肝硬化 病死率 

分 类 号：R374[医药卫生—病原生物学]