二氧化碳超临界流体萃取两面针根挥发油成分的气相色谱-质谱分析  

GC-MS Analysis of Essential Oil from the Roots of Zanthoxylum Nitidum DC. Extracted by Supercritical CO_2 Fruid

作　　者：周劲帆[1] 覃富景[1] 冯洁[1] 赖茂祥[2] 

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西中医药研究院,广西南宁530022

出　　处：《中国药业》2012年第11期5-6,共2页China Pharmaceuticals

基　　金：广西自然科学基金项目;项目编号:2010GXNSFA013187;广西医科大学博士启动资金资助;广西中医药管理局中医药专项;项目编号:GZKZ1143

摘　　要：目的分析两面针根挥发油的化学成分,为两面针药材的综合开发应用提供科学实验依据。方法采用二氧化碳超临界流体萃取两面针根挥发油成分,用气相色谱毛细管柱进行分离,质谱检测,归一化法测定其相对含量。结果检出34个成分,鉴定了23个化合物,占挥发油总量的95.12%。两面针根的挥发油主要化学成分为萜类,其中荜澄茄烯醇相对含量高达49.96%,其次是亚麻油酸和亚油酸,含量分别为14.34%和10.16%。结论所用方法所得结果与文献报道的差异较大,可能与产地、采收期、提取方法等因素有关。Objective To analyze the constituents of the essential oil extracted from the roots of Zanthoxylum nitidum （Roxb.） DC. , and provide scientific foundations of comprehensive usage. Methods The essential oil was extracted by supereritieal CO2 fruid and identified by GC- MS. The relative content was determined by normalization method. Results Thirtyfour compounds were detected and 23 compounds were identified which were 95.12% of the total essential oil. The terpenes were the main constituents in the essential oil extracted from the roots of Zanthoxylum nitidum. Among them, cubenol was 49.96% of the total essential oil, and （Z, Z）-9, 12- octadecadienoic acid and oleic acid were 14. 34% and 10. 16%, respectively. Conclusion The results are different from the references. It may be related to the factors as location, harvest time and extraction method.

关 键 词：两面针根 挥发油 化学成分 气相色谱-质谱分析 二氧化碳超临界流体萃取 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]