潮州地区乙肝病毒分型和耐药突变基因检测的应用  

Application of hepatitis B virus genotyping and drug-resistance mutation genes detecting in Chaozhou area

作　　者：陈立华[1] 黄泽伟[1] 罗招云[1] 林芬[1] 林春萍[1] 

机构地区：[1]广东省潮州市中心医院检验实验中心,521021

出　　处：《中国误诊学杂志》2012年第14期3444-3446,共3页Chinese Journal of Misdiagnostics

基　　金：广东省医学科研基金项目（A2011760,B2008179）

摘　　要：目的探讨潮州地区乙型肝炎病毒（HBV）基因分型和耐药基因突变检测的临床意义。方法采用PCR-反向点杂交（RDB）法对慢性乙肝（ChronichepatitisB，CHB）患者血清进行基因分型和耐药突变检测，拉米夫定（Lamivudine）耐药患者改用阿德福韦酯（Adefovir）治疗，1a后测定其耳BVDNA和谷丙转氨酶（ALT）含量。结果HBVB亚型占92．31％（120／130），HBVC亚型占5．38％（7／130），B＋D基因型占3．21％（3／130）。检测出24例均为HBVB亚型的耐药突变基因，耐药突变率18．46％（24／130）。HBVB亚型与HBVC亚型的HBVDNA和ALT含量比较差异有统计学意义（P〈O．05），阿德福韦酯治疗1a前后的HBVDNA和ALT含量差异有统计学意义（P〈0．05）。结论潮州地区HBV基因型以B亚型为主，耐药突变基因型是影响疾病进程的重要因素。Objective To evaluate the clinical significance of hepatitis B virus genotype and drug resistance genes mu- ration detection in Chaozhou. Methods The PCR-RDB was used to detect hepatitis B virus genotypes and drug re- sistance genes mutations in CHB patients. Adefovir was selected to therapy the patients with Lamivudine resistance gene mutations,the HBV DNA load and ALT content were detected again one year later. Results The infection rate of HBV B,C and B＋D were 92.31 % （120/130} ,5.38% （7/130} ,3.21% 13/130） respectively. Lamivudine resistance mutation genes were detected in 24 persons,who were all HBV B genotype infectors. The rate of drug-resistance mu- tation was 18.46 %（24/130）. Significant differences were found between the HBV B and the HBV C infectors when their HBV DNA load and ALT content were compared. Moreover, the HBV DNA load and ALT content of the Ade- fovir receptor were significantly decreased than one year ago. Conclusions HBV B is the most common HBV geno- type in Chaozhou. Drug-resistance genes mutation is one of key factor that influence the CHB development.

关 键 词：肝炎病毒 乙型/遗传学 药物耐受性/遗传学 

分 类 号：R373.21[医药卫生—病原生物学]