慢性血吸虫病患者结肠腺磷酸糖蛋白表达及临床意义  

Expression and clinical significance of P-gp in colonic gland of chronic schistosomiasis patients

作　　者：冯林森[1] 段艳萍[2] 吴泽生[3] 李靖娟[4] 吴皎[1] 张爱芬[1] 张东葵[2] 王金德[5] 

机构地区：[1]昆明医科大学第一临床学院昆明650031 [2]昆明医科大学组织学与胚胎学教研室 [3]大理学院附属医院消化内科 [4]昆明医科大学第二临床学院 [5]昆明医科大学人体解剖学教研室

出　　处：《中国血吸虫病防治杂志》2012年第3期290-293,F0003,共5页Chinese Journal of Schistosomiasis Control

基　　金：云南省教育科学规划课题(GY09006);昆明医科大学2011年大学生创新性实验计划资助课题(CX201172)

摘　　要：目的探讨慢性血吸虫病患者磷酸糖蛋白(P gp)的表达情况及其与性别、年龄的关系,并分析其临床意义。方法选取50例慢性血吸虫病患者及4例有结肠癌家族史但体检正常者的结肠黏膜标本,采用免疫组织化学染色法检测P gp在结肠腺中的表达情况。结果与正常对照者相比,慢性血吸虫病患者结肠腺上皮细胞P gp阳性颗粒平均灰度值明显降低(P<0.05),阳性单位值明显增加(P<0.05),但慢性血吸虫病患者结肠腺上皮细胞P gp表达量增加与性别及年龄无关。结论慢性血吸虫病可诱导结肠腺上皮细胞P gp表达量增加,这可能是机体对血吸虫卵长期刺激结肠组织产生的一种代偿性保护机制。Objective To study the expression of P-glycoprotein （P-gp） in patients with chronic schistosomiasis and its rela- tionship with gender and age, and to explore its clinical significance. Methods The colonic mucosa specimens of 50 chronic schistosomiasis patients and 4 persons who had a family history of colon cancer but their physical examinations were normal （as a control group） were selected and the expressions of P-gp in the colonic gland of these samples were detected with the immunohisto- chemical staining method. Results Compared with the control group, the mean gray value of the positive panicles of P-gp of co- lonic epithelial ceils of the chronic schistosomiasis group was significantly reduced （P 〈 0.05 ）, but positive unit values were signif- icantly increased （P 〈 0.05）, which meant the P-gp expression in colonic gland of the patients with chronic schistosomiasis was significantly increased, but this increase had no relationship with sex and age. Conclusion Chronic schistosomiasis may induce the increase of P-gp expression which may be a compensatory protection mechanism due to the stimulation of schistosome eggs to the colon tissues.

关 键 词：慢性血吸虫病 结肠腺 磷酸糖蛋白 

分 类 号：R532.21[医药卫生—内科学]