高效液相色谱-四级杆飞行时间串联质谱分析3种荷花的花色苷  

Analysis of anthocyanins in three species Nelumbinis flos. by HPLCElectrospray Ionization/Quadrupole-Time of Flight-Mass/Mass Spectrometry

作　　者：徐双双[1] 刘伟[1] 郭英慧[2] 王萍[2] 孙瑜[1] 刘建华[1] 王晓[1] 

机构地区：[1]山东省分析测试中心,山东济南250014 [2]山东中医药大学基础医学院,山东济南250355

出　　处：《山东科学》2012年第3期66-70,共5页Shandong Science

基　　金：作物生物学国家重点实验室开放课题(20082KF05);山东省科技攻关项目(2010GSF10287)

摘　　要：应用高效液相色谱-电喷雾/四极杆飞行时间串联质谱联用技术(HPLC-ESI/Q-TOF-MS/MS)分析了3种荷花(大红袍、洪湖红莲、红牡丹)的花色苷成分。首先以φHCl=0.1%的甲醇超声提取荷花中的花色苷,然后用XAD-7大孔树脂纯化,选用Amethyst C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-水(φC2HF3O2=0.05%)为流动相进行梯度洗脱,柱后流出液采用电喷雾四极杆飞行时间质谱(ESI/Q-TOF-MS/MS)进行检测,共分离鉴定出6种花色苷,并且运用pH示差法分别测定了3种荷花中总花色苷的含量,为开发利用这一丰富的自然资源提供一定的参考依据。We employed HPLC-Electrospray Ionization/Quadrupole-Time of Flight-Mass/Mass Spectrometry （ HPLC-ESI/ Q-TOF-MS/MS） to analyze the anthocyanins in three species Nelumbo nucifera. The anthocyanins were extracted with methanol containing HCI （φFacl = 0. 1%） and then purified on an Amberlite XAD-7 column. We applied an Amethyst C18 column （250 mm ×4.6 mm, 5 μm） to HPLC analysis with water methanol-water （containing trifluoroacetic acid （φC1HF3O3 = 0.0.5% ） ） in gradient as mobile phase. The eluates were then detected by ESI/Q-TOF-MS/MS. We altogether identified six s.pecies anthocyanins from these Nelumbo nucifera. We further determined their contents by pH-differential spectrophotometry. It provides theoretical foundation for further development and utilization of Nelumbo nucifera resources.

关 键 词：高效液相色谱-四级杆飞行时间串联质谱 荷花 花色苷 

分 类 号：O657.63[理学—分析化学]