模版合成DNA纳米管:用于基因和药物输送纳米新技术  

Template synthesis of DNA nanotube:-New gene and drug delivery vehicle

作　　者：侯士峰 朱军[2] 王宁[2] 杨婕[2] 

机构地区：[1]蒙特克莱尔州立大学化学系,美国新泽西07043 [2]济宁医学院生物纳米技术与医学工程研究中心,山东济宁272067

出　　处：《济宁医学院学报》2012年第3期162-167,共6页Journal of Jining Medical University

基　　金：国家自然科学基金的支持(基金号:21175059)

摘　　要：利用氧化铝多孔膜作为模板合成了DNA纳米管,DNA纳米管由单链DNA片段杂交在氧化铝多孔膜内以DNA双链杂交组成;药物分子和DNA片段相连后,可把药物分子包覆于DNA纳米管中。加热时,双链DNA纳米管分解,释放出单链DNA分子,以此可实现基因和药物分子的可控输送和释放。DNA nanotubes were fabricated within the anodisc alumina membrane（AAM）by utilizing layer-by-layer technique.The wall of nanotube is composed of DNA,and the layers are held together by hybridization of complementary single-stranded DNA oligonucleotides（ss-DNA）.Ss-DNA can be released from the DNA nanotubes by melting of the duplexes.It was found that DNA sequence,DNA layers will affect the release temperature and released amounts.Fluorescence dyes are linked to ss-DNA and are used to construct the DNA nanotubes and to simulate drug delivery process.

关 键 词：DNA纳米管 药物可控输送 

分 类 号：R445.1[医药卫生—影像医学与核医学]