Optical control after transfection of channelrhodopsin-2 recombinant adenovirus in visual cortical cells  

作　　者：Junping Yao Wensheng Hou Hao Wang Hui Liu Chuanhuang Weng Zhengqin Yin 

机构地区：[1]College of Bioengineering,Chongqing University,Chongqing 400044,China [2]Chongqing Key Lab of Visual Damage and Regeneration&Restoration,Chongqing 400038,China [3]Department of Ophthalmology,Southwest Hospital,Third Military Medical University of Chinese PLA,Chongqing 400038,China

出　　处：《Neural Regeneration Research》2012年第16期1228-1233,共6页中国神经再生研究（英文版）

基　　金：sponsored by the National Natural Science Foundation of China (General Program),No.81070749,31070882,30970758;Key Project of Chongqing Science & Technology Commission,No. 2010AB5118

摘　　要：Channelrhodopsin-2 ectopically expressed in the retina can recover the response to blue light in genetically blind mice and rats, but is unable to restore visual function due to optic nerve or optic tract lesions. Long Evans rats at postnatal day 1 were used for primary culture of visual cortical cells and 24 hours later, cells were transfected with recombinant adenovirus carrying channelrhodopsin-2 and green fluorescent protein genes. After 2 4 days of transfection, green fluorescence was visible in the cultured cells. Cells were stimulated with blue light （470 nm）, and light-induced action potentials were recorded in patch-clamp experiments. Our findings indicate that channelrhodopsin-2-recombinant adenovirus transfection of primary cultured visual cortical cells can control the production of action potentials via blue light stimulation.Channelrhodopsin-2 ectopically expressed in the retina can recover the response to blue light in genetically blind mice and rats, but is unable to restore visual function due to optic nerve or optic tract lesions. Long Evans rats at postnatal day 1 were used for primary culture of visual cortical cells and 24 hours later, cells were transfected with recombinant adenovirus carrying channelrhodopsin-2 and green fluorescent protein genes. After 2 4 days of transfection, green fluorescence was visible in the cultured cells. Cells were stimulated with blue light （470 nm）, and light-induced action potentials were recorded in patch-clamp experiments. Our findings indicate that channelrhodopsin-2-recombinant adenovirus transfection of primary cultured visual cortical cells can control the production of action potentials via blue light stimulation.

关 键 词：channelrhodopsin-2 recombinant adenovirus visual cortex blue light action potentials neuralregene ration 

分 类 号：Q782[生物学—分子生物学] Q959.117.3