Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury  

作　　者：Junliang Li Xinke Xu Shanyi Zhang Meiguang Zheng Zhonghua Wu Yinlun Weng Leping Ouyang Jian Yu Fangcheng Li 

机构地区：[1]Department of Neurosurgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China [2]Department of Neurosurgery, Henan Provincial People＇s Hospital, Zhengzhou 450003, Henan Province, China

出　　处：《Neural Regeneration Research》2012年第17期1338-1342,共5页中国神经再生研究（英文版）

基　　金：funded by the National Natural Science Foundation of China,No. 30770765

摘　　要：In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury. The results of flow cytometry and TUNEL showed that exogenous glucose transporter 3 significantly suppressed PC12 cell apoptosis induced by ischemic injury. The results of isotopic scintiscan and western blot assays showed that, the glucose uptake rate was significantly increased and nuclear factor kappaB expression was significantly decreased after adenovirus-mediated transfection of ischemic PC12 cells with glucose transporter 3. These results suggest that adenovirus-mediated transfection of cells with glucose transporter 3 elevates the energy metabolism of PC12 cells with ischemic injury, and inhibits cell apoptosis.In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury. The results of flow cytometry and TUNEL showed that exogenous glucose transporter 3 significantly suppressed PC12 cell apoptosis induced by ischemic injury. The results of isotopic scintiscan and western blot assays showed that, the glucose uptake rate was significantly increased and nuclear factor kappaB expression was significantly decreased after adenovirus-mediated transfection of ischemic PC12 cells with glucose transporter 3. These results suggest that adenovirus-mediated transfection of cells with glucose transporter 3 elevates the energy metabolism of PC12 cells with ischemic injury, and inhibits cell apoptosis.

关 键 词：glu-cose t ansporter 3 ischemia glucose uptake rate apoptosis nuclear factor kappaB 

分 类 号：Q532.3[生物学—生物化学] Q51