胸椎黄韧带骨化症与骨形态发生蛋白4基因单核苷酸多态性的关联  

Bone morphogenetic protein-4 gene polymorphism associated with ossification of the thoracic ligamentum flavum

作　　者：赵伟光[1] 刘振武[1] 刘利[1] 谢延平[1] 江丽强[1] 林欣[2] 

机构地区：[1]邯郸市中心医院骨一科,河北省邯郸市056001 [2]首都医科大学附属北京天坛医院骨科,北京市100050

出　　处：《中国组织工程研究》2012年第24期4376-4380,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(30872599);北京市自然科学基金(7092028)~~

摘　　要：背景:骨形态发生蛋白4基因与胸椎黄韧带骨化症的相关性研究目前较少。目的:测观察骨形态发生蛋白4的2个单核苷酸多态性位点rs17563和rs2855532与胸椎黄韧带骨化症的关联。方法:胸椎黄韧带骨化症患者和正常对照者各40例,收集受试者的周围静脉血提取DNA,PCR法进行目的片段骨形态发生蛋白4的单核酸多态性位点rs17563和rs2855532的扩增并测序。结果与结论:黄韧带骨化症组中rs17563和rs2855532位点带"T"基因型及等位基因型频率明显高于对照组(P<0.05)。证实,骨形态发生蛋白4上的2个单核酸多态性位点rs17563和rs2855532等位基因型突变与胸椎黄韧带骨化症的发生有关。BACKGROUND： There are few studies addressing the association of bone morphogenetic protein-4 gene and ossification of the thoracic ligamentum flavum （OLF）. OBJECTIVE： To explore whether two single nucleotide polymorphisms of the bone morphogenetic protein-4 were related to the morbidity and severity of OLF. METHODS： The venous blood samples of 40 OLF patients and 40 non-OLF controls were collected. All experimental participants were matched in age-sex composition. The DNA extracted from the peripheral venous blood was amplified and sequenced at two sites of rs17563 and rs2855532 by using PCR. RESULTS AND CONCLUSION： The frequency of ＂T＂ genotype and allelotype at rs17563 and rs2855532 in the OLF patients was higher than that in the non-OLF patients （P 〈 0.05）. The two single nucleotide polymorphisms （rs17563 and rs2855532） can increase the incidence of OLF,

关 键 词：黄韧带骨化症 胸椎 骨形态发生蛋白4 单核苷酸多态性 聚合酶链反应 病例-对照研究 组织构建 

分 类 号：R318[医药卫生—生物医学工程]