不同浓度血管抑素抑制大鼠角膜血管的新生  

Different-concentration angiostatin inhibits rat corneal neovascularizasion

作　　者：刘子彬[1] 刘海俊[2] 封亮旗[1] 魏二霞[1] 许丹丹[1] 

机构地区：[1]南方医科大学,广东省广州市510515 [2]解放军第421医院,广东省广州市510318

出　　处：《中国组织工程研究》2012年第24期4427-4431,共5页Chinese Journal of Tissue Engineering Research

基　　金：广东省自然科技资源建设项目(20090601)~~

摘　　要：背景:血管抑素在角膜中是否抑制组织中的新生血管生长尚不清楚。目的:观察血管抑素溶液对大鼠角膜新生血管生长的作用。方法:24只大鼠制备左眼碱烧伤角膜新生血管模型,随机抽签法分为4组,对照组给予生理盐水,25,50,75mg/LAS组分别给与25,50,75mg/LAS血管抑素溶液滴眼,4次/d至实验结束。结果与结论:造模后第3天各组角膜均可见少量血管新生,但对照组角膜新生血管生长较其他3组明显。3～7d,各组角膜新生血管生长速度加快,对照组角膜新生血管粗大。烧伤后14d,对照组角膜新生血管仍然粗大未见明显消退,各浓度组较前消退明显。碱烧伤后不同时间点各浓度组角膜新生血管面积较对照组少(P<0.05),75mg/LAS组碱烧伤14d各时间新生血管面积较其他3组减少(P<0.05)。Real-TimePCR结果显示:各浓度组间CD31mRNA表达差异均有显著性意义(P<0.01)。Westernblot结果显示血管抑素作用大鼠后,各浓度组角膜组织的CD31表达均较对照组降低,且随着血管抑素溶液浓度的增加而减少。提示血管抑素能够阻止角膜新生血管的生长。BACKGROUND： It is unclear whether angiostatin inhibits tissue neovascularization OBJECTIVE： To explore the effect of angiostatin on rat corneal neovascularization. METHODS： Corneal neovascularization models were established in 24 rats after alkali burns, which were randomly divided into four groups： control group treated with normal saline, and three experimental groups treated with different-concentration angiostatin solution （25, 50, 75 mg/L）, four times per day. RESULTS AND CONCLUSION： There were few new vessels in the four groups at 3 days after modeling, and corneal neovascularization appeared obviously in the control group as compared with other three groups. At 3-7 days, the speed of corneal neovascularization accelerated, and new vessels in the control group were thicker compared with other groups The area of corneal neovascularization in the three experimental groups was less than that in the control group after alkali burns （P 〈 0.05）. 75 mg/L angiostatin drop group had the smallest neovascularized corneal area （P 〈 0.05）. Real-time PCR results showed that there was a significant difference in the mRNA expression of CD31 among different groups （P 〈 0.01 ）. Western blot findings demonstrated that the CD31 expression was lower in the three experimental groups than the control group, and lowered with the increase of angiostatin concentrations, indicating that the angiostatin eye drops can inhibit the coreal neovascularization.

关 键 词：角膜 新生血管 血管抑素 CD31 组织构建 

分 类 号：R318[医药卫生—生物医学工程]