纯化蛆虫分泌物抗菌肽对糖尿病大鼠溃疡创面的抗菌作用  

Antibacterial effect of purified maggot secretion antimicrobial peptides on ulcer wound of diabetic rats

作　　者：高磊[1] 尹叶锋[1] 王寿宇[2] 王江宁[1,3] 

机构地区：[1]大连大学,辽宁省大连市116622 [2]大连医科大学附属第一医院骨科,辽宁省大连市116011 [3]首都医科大学北京潞河医院,北京市101100

出　　处：《中国组织工程研究》2012年第24期4437-4440,共4页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81071586)~~

摘　　要：背景:蛆虫的分泌物对感染创面具有良好的抗菌作用,经课题组研究证实分泌物中有抑制细菌和杀灭细菌的成分抗菌肽,并对该抗菌肽进行了有效纯化。目的:观察纯化蛆虫分泌物抗菌肽对糖尿病大鼠溃疡创面的抗菌和促愈合作用。方法:取3.5月龄雄性SD大鼠20只,体质量330～370g,制备糖尿病大鼠溃疡创面模型。随机均分为2组,实验组创面涂以纯化的蛆虫分泌物抗菌肽,对照组不予处理。结果与结论:实验组创面清洁,新鲜肉芽生长,无脓性分泌物,愈合情况良好,无金色葡萄球菌感染;对照组创面渗出、糜烂严重,创面不断加大加深,愈合情况不良,金色葡萄球菌感染率为70%。术后7,14,21,28d,实验组溃疡面积明显小于对照组,差异均有显著性意义(P<0.05)。结果表明纯化的蛆虫分泌物抗菌肽有效促进糖尿病大鼠溃疡创面愈合,预防组织细菌感染。BACKGROUND： Maggot secretions have a good antibacterial effect on infected wounds. The studies have confirmed that there are antimicrobial peptides that can inhibit and kill the bacteria in the maggot secretions, and we have carried out effective purification of the peptide. OBJECTIVE： To investigate the antibacterial effect and wound healing of purified maggot secretion antimicrobia peptides on ulcer wound of diabetic rats. METHODS： Twenty 3.5-month-old male SD rats weighing 330 370 g was used to prepare diabetic ulcer wound model. The rats were randomly divided into two groups （n=10）. In the experimental group, the wounds were coated with purified maggot secretion antimicrobial peptide; the control group was not treated. RESULTS AND CONCLUSION： In the experimental group, the wounds were clean and healed well with fresh granulation, no purulent secretions and no staphylococcus aureus infections. In the control group, wound exudates were found with severe erosion, and the wound was enlarged and deepened that healed poor. The staphylococcus aureus infection rate in the control group was 70%. After 7, 14, 21 and 28 days, the ulcer area in the experimental group was significantly smaller than that in the control group （P 〈 0.05）. It indicates that the purified maggot secretion antimicrobial peptide can promote the ulcers wound healing of diabetic rats and prevent the tissue bacterial infection.

关 键 词：蛆虫分泌物 糖尿病 溃疡组织 抗菌肽 抗菌作用 

分 类 号：R318[医药卫生—生物医学工程]