实验性牙髓炎模型兔的构建  

Construction of experimental pulpitis models in rabbits

作　　者：毛小泉[1] 邢璐[2] 谈顺[2] 吴多荣[3] 徐普[1] 黄茜[1] 

机构地区：[1]中南大学湘雅医学院附属海口医院海南省口腔医学中心,海南省海口市570208 [2]中南大学湘雅医学院附属海口医院病理科,海南省海口市570208 [3]中南大学湘雅医学院附属海口医院中心实验室,海南省海口市570208

出　　处：《中国组织工程研究》2012年第24期4467-4470,共4页Chinese Journal of Tissue Engineering Research

基　　金：海南省自然科学基金资助项目(807091)~~

摘　　要：背景:以往牙髓炎动物模型的建立大多应用内毒素,不能完全反映临床牙髓炎的形成过程。目的:观察应用变形链球菌诱导兔牙牙髓炎模型不同时间炎症特征。方法:选用20只健康新西兰大白兔,随机将下颌前牙分为实验组和对照组;在兔两颗下颌前牙舌面钻孔,对照组一颗置入生理盐水,实验组另一颗置入变形链球菌,术后1,3,5,7d各处死兔5只;观察牙髓炎炎症转归。结果与结论:实验组术后1d炎症反应较轻,经过3,5,7d牙髓炎症反应逐渐加重,牙髓血管充血、渗出严重,牙髓组织出现坏死。对照组牙髓炎症随着时间的推移,血管扩张逐渐加重,偶有牙髓组织坏死。结果表明,应用变形链球菌诱导牙髓炎症形成过程更符合牙髓炎病理学特征。BACKGROUND： Animal models of pulpitis have been established using endotoxin in the past; however, they cannot fully reflect the formation process of clinical pulpitis. OBJECTIVE： To observe the inflammatory characteristics at different time points of rabbit models of pulpitis induced by Streptococcus mutans. METHODS： The mandibular anterior teeth of 20 healthy New Zealand rabbits were divided into experiment group and control group. The lingual surfaces of the rabbit mandibular anterior teeth were drilled; normal saline was imported into the control tooth, while Streptococcus mutans was imported into the experiment tooth. Five rabbits were sacrificed on 1, 3 5 and 7 days after operation, respectively; inflammation outcomes of pulpitis was observed. RESULTS AND CONCLUSION： Inflammatory reaction in the experiment group was slight on 1 day after operation, and was aggravated gradually on 3, 5 and 7 days after operation, accompanied with vascular hyperemia in the pulp, severe effusion and necrosis of the pulp tissue. Inflammation in the control group was aggravated gradually as time went on; very few necrosis were found in the pulp tissues. These findings indicate that the forming process of pulpitis induced by Streptococcus mutans is more suitable to the pathological features of pulpitis.

关 键 词：牙髓炎 动物模型 炎症 兔 组织学 

分 类 号：R318[医药卫生—生物医学工程]