蓬草除痹汤干预类风湿性关节炎大鼠骨吸收骨重建及细胞因子的表达  

Effect of Peng Cao rheumatism-syndrome dispelling decoction on bone resorption,bone remodeling and cytokine expression in rats with rheumatoid arthritis

作　　者：刘春景[1] 田新玮[1] 王学涵[1] 徐迪[1] 

机构地区：[1]新疆医科大学第五附属医院,新疆维吾尔自治区乌鲁木齐市830011

出　　处：《中国组织工程研究》2012年第24期4499-4504,共6页Chinese Journal of Tissue Engineering Research

基　　金：新疆医科大学科研创新基金资助项目(SJC201073)~~

摘　　要：背景:实验研究显示,复方中药能有效下调RANKL或上调护骨素及调节RANKL/OPG轴而发挥中药抑制关节破坏和骨吸收的作用。目的:进一步验证中药蓬草除痹汤对类风湿性关节炎大鼠细胞因子及骨吸收和重建的影响。方法:取Wistar大鼠60只,随机分为6组,空白组、模型组、中药蓬草除痹汤高、中、低剂量组、雷公藤对照组。空白组不做处理,其余大鼠建立胶原诱导性关节炎大鼠模型,模型组不做处理,其他各组均为灌胃给药,1次/d,连续给药28d。结果与结论:与模型组比较,各治疗组可以显著抑制大鼠右足肿胀的程度(P<0.01或P<0.05)。各治疗组血清白细胞介素1β、肿瘤坏死因子α及前列腺素E2水平均明显低于模型组(P<0.01),其中蓬草除痹汤中、高剂量组抑制作用明显优于雷公藤多甙片组(P<0.05)。各治疗组大鼠关节软骨下骨和骨小梁区核因子κB受体活化因子配体表达均低于模型组(P<0.01),护骨素关节软骨组织表达量均低于模型组(均P<0.01)。表明,蓬草除痹汤能有效改善胶原诱导性关节炎大鼠关节炎症状,减轻骨侵蚀,其机制可能与下调血清中致炎因子白细胞介素1β、肿瘤坏死因子α及前列腺素E2的水平,抑制类风湿性关节炎大鼠关节中核因子κB受体活化因子配体表达有关。BACKGROUND： Experimental studies have shown that the compound Chinese medicine can effectively lower receptor activator of nuclear factor kappa B ligand or up-regulate osteoprotegerin and regulate receptor activator of nuclear factor kappa B ligand/osteoprotegerin to inhibit joint damage and bone resorption. OBJECTIVE： To observe Peng Cao rheumatism-syndrome dispelling decoction＇s effect on cytokines, bone resorption and remodeling of rats with rheumatoid arthritis. METHODS： Sixty Wistar rats were randomized into six groups： blank group, model group, high-, middle- and low- dosage Peng Cao rheumatism-syndrome dispelling decoction groups and Tripterygium wilfordii group. Except blank and model group, other groups were administrated intragastrically once a day for 28 days. RESULTS AND CONCLUSION： Compared with the model group, other groups had a remarkable effect on relieving right foot swelling （P 〈 0.01 or 0.05）. The levels of serum interleukin 113, prostaglandin E2, tumor necrosis factor a were significantly lower in the treatment groups than the model group （P 〈 0.01 ）. The middle- and high-dose Peng Cao rheumatism-syndrome dispelling decoction had a better effect than Tripterygium wilfordii （P 〈 0.05）. The expressions of receptor activator of nuclear factor kappa B and osteoprotegerin were significantly lower in the treatment groups than the model group （P 〈 0.01 ）. These findings indicate that Peng Cao rheumatism-syndrome dispelling decoction is capable of improving symptoms of rheumatoid arthritis effectively and restraining bone erosion. The mechanism may relate to the following aspects： （1） Reduction in levels of interleukin 113, prostagtandin E2, tumor necrosis factor a in serum; （2） Expression restraint on receptor activator of nuclear factor kappa B ligand in the joint of rats with rheumatoid arthritis.

关 键 词：胶原诱导性关节炎大鼠模型 中药 白细胞介素1Β 肿瘤坏死因子α 前列腺素E2 

分 类 号：R318[医药卫生—生物医学工程]