动脉粥样硬化模型兔血管平滑肌细胞增殖与癫狂梦醒汤的干预  

Effects of Dian Kuang Meng Xing Tang on the proliferation of vascular smooth muscle cells in atherosclerosis rabbits

作　　者：韩凤丽 于铭权 

机构地区：[1]内蒙古莫旗人民医院,内蒙古自治区呼伦贝尔市162850

出　　处：《中国组织工程研究》2012年第24期4509-4513,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:现代临床研究表明癫狂梦醒汤能够治疗神经官能症、更年期综合征、癔病、老年痴呆等精神系统疾病。目的:观察癫狂梦醒汤对动脉粥样硬化模型兔血管平滑肌细胞增殖的影响。方法:将32只日本大耳白兔随机分成空白对照组、模型组、癫狂梦醒汤组、辛伐他汀组,分别给予普通饲料、高脂饲料、高脂饲料+癫狂梦醒汤、高脂饲料+辛伐他汀进行干预,喂养至第12周末。结果与结论:喂养高脂饲料成功建立动脉粥样硬化模型,模型组有严重的动脉粥样硬化病理改变,增殖细胞核抗原、血小板源生长因子B蛋白表达明显升高,平滑肌细胞呈合成型改变;癫狂梦醒汤组及辛伐他汀组动脉粥样硬化病变明显减轻,增殖细胞核抗原、血小板源生长因子B蛋白表达明显下降(P<0.01),平滑肌细胞接近"收缩型"改变。结果可见癫狂梦醒汤可抑制血管平滑肌细胞增殖,延缓动脉粥样硬化发生发展,这一作用可能是通过抑制增殖细胞核抗原、血小板源生长因子B蛋白表达来实现的。BACKGROUND： Modern clinical studies have shown madness decoction） has remedial effects on neurosis disorders. that Dian Kuang Meng Xing Tang （awaken from the dream of menopausal syndrome, hysteria, dementia and other mental OBJECTIVE： To investigate the effects of Dian Kuang Meng Xing Tang on the proliferation of vascular smooth muscle cells in atherosclerosis rabbits. METHODS： A total of 32 Japan big-ear white rabbits were randomly divided into normal control group, model group, Dian Kuang Meng Xing Tang group and Simvastatin group; the corresponding rabbits in each group were fed with normal diet, high fat diet, high fat diet＋ Dian Kuang Meng Xing Tang and high fat diet＋Simvastatin for 12 weeks, respectively. RESULTS AND CONCLUSION： The atherosclerosis model was successfully established with high fat diet. There was a severe pathological lesion of atherosclerosis in the model group; the expression of proliferating cell nuclear antigen and platelet-derived growth factor B was significantly increased, the smooth muscle ceils were synthetically changed. The pathological lesion of atherosc^erosis in the Dian Kuang Meng Xing Tang group and Simvastatin group were significantly alleviated; the expression of proliferating cell nuclear antigen and platelet-derived growth factor B were significantly reduced; the change of smooth muscle cells were close to ＂contractile＂ change. These findings indicate that Dian Kuang Meng Xing Tang has an inhibit effect on the proliferation of smooth muscle cells and can delay the development of atherosclerosis by inhibiting the expression of proliferating cell nuclear antigen and platelet-derived growth factor B.

关 键 词：癫狂梦醒汤 血管平滑肌细胞 增殖细胞核抗原 血小板源性生长因子B 动脉粥样硬化 

分 类 号：R318[医药卫生—生物医学工程]