骨基质明胶/煅烧骨基质重组人工骨对骨髓间充质干细胞成骨潜能的影响  

Effect of bone matrix gelatin/true bone ceramic on the osteogenic potential of bone marrow mesenchymal stem cells

作　　者：刘喜民[1] 田志逢[1] 张小玲[2] 王丰刚[1] 

机构地区：[1]漯河医学高等专科学校解剖教研室,河南省漯河市462002 [2]漯河医学高等专科学校病理生理教研室,河南省漯河市462002

出　　处：《中国组织工程研究》2012年第25期4566-4570,共5页Chinese Journal of Tissue Engineering Research

基　　金：漯河医学高等专科学校2009年校级自然课题(2009-LMC-S09);课题名称:骨基质明胶与煅烧骨基质重组人工骨的研究~~

摘　　要：背景:前期实验发现单纯骨基质明胶材料与骨髓间充质干细胞复合后具有较好的成骨能力,修复骨缺损效果较好,但单纯骨基质明胶降解速度快,硬度差,不能用来修复承重区域的骨缺损。目的:观察骨基质明胶/煅烧骨基质重组人工骨与骨髓间充质干细胞复合培养后细胞成骨潜能的变化。方法:利用骨基质明胶和煅烧骨基质制备重组人工骨,并与大鼠骨髓间充质干细胞复合培养48h,用茜素红染色检测复合培养后细胞的成骨潜能。结果与结论:细胞在复合材料上能够很好的黏附、生长以及增殖,与重组人工骨复合培养后,细胞的成骨潜能和细胞表型无明显变化。表明重组人工骨易于与骨髓间充质干细胞结合,对细胞成骨潜能无影响,生物相容性良好。BACKGROUND： Previous studies have found that simply bone matrix gelatin （BMG） material,compound with bone marrow mesenchymaL stem cells （BMSCs） has a good osteogenic potential for bone defects with better effects, but simply BMG cannot be used to repair bone defects in load-bearing area because of rapid degradation speed and bad hardness. OBJECTIVE： To observe the changes in the osteogenic potential of BMSCs co-cultured with BMG/true bone ceramic （BMG/TBC）. METHODS： BMG and TBC were used to prepare recombinant bone that was co-cultured with BMSCs for 48 hours in vitro Osteogenic potential of the recombinant bone was detected by Alizarin bordeaux staining. RESULTS AND CONCLUSION： BMSCs in composited material could adhere, grow and proliferate well after co-cultured with BMG/TBC. There were no changes of the osteogenic potential and cell phenotype. It is indicated that BMG/TBC is easy to combined with BMSCs without affecting osteogenic potential, which shows a good biocompatibility.

关 键 词：骨基质明胶 煅烧骨基质 骨髓间充质干细胞 成骨 人工骨 生物材料 

分 类 号：R318[医药卫生—生物医学工程]