不同植骨材料诱导兔腰椎横突间的融合  

Different bone graft materials induce the lumbar intertransverse process fusion in rabbits

作　　者：孙君超[1] 肖荣驰[1] 欧俊[2] 江玉民[2] 孙煦化[1] 

机构地区：[1]桂林医学院附属医院骨一科,广西壮族自治区桂林市541001 [2]桂林理工大学,广西壮族自治区桂林市541001

出　　处：《中国组织工程研究》2012年第25期4586-4590,共5页Chinese Journal of Tissue Engineering Research

基　　金：广西卫生厅重点课题(重2010056)~~

摘　　要：背景:生物玻璃作为骨修复植入体可以在材料界面与人体骨组织之间形成化学键合,可诱导骨的修复与再生,但是其抗弯强度不足,引起的应力屏蔽可造成骨修复失败。目的:评价不同植骨材料对兔腰椎横突间融合的影响,探讨其替代自体骨用于腰椎横突间融合的可行性。方法:将健康成年雄性新西兰大白兔28只随机均分4组,壳聚糖/硅酸钙组,硅酸钙组,壳聚糖组,自体髂骨组。分别行腰椎后外侧横突间脊柱融合术,将植入材料植入两侧的L4/5横突间。结果与结论:术后12周,大体及手法检测发现壳聚糖/硅酸钙组7例均融合,硅酸钙组、壳聚糖组和自体髂骨组分别为4例,4例和5例;组织学观察显示壳聚糖/硅酸钙组材料对于降解、成骨有显著促进作用;生物力学检测结果表明,壳聚糖/硅酸钙组抗拉伸强度优于其他组(P<0.05)。结果显示3种材料均能代替自体骨行脊柱横突间融合,壳聚糖/硅酸钙复合材料更能促进脊柱融合,并能提高脊柱融合的生物力学强度。BACKGROUND ： Chemical bonding can be formed between the material interface and human bone after implantation of bioactive glass as bone graft into human body. Bioactive glass can induce bone repair and regeneration, but its poor bending strength may lead to stress shielding and failure of bone repair. OBJECTIVE： To observe the effects of different bone graft materials on lumbar intertransverse fusion of rabbits, and to investigate their feasibilities to replace autologous bone in lumbar intertransverse fusion. METHODS： Twenty-eight healthy male adult New Zealand white rabbits were randomly divided into four groups： chitosan/ calcium-silicate group, calcium-silicate group, chitosan group and autologous ilium group. Lumbar posterolateral fusion was performed in all rabbits to place the implant materials into both sides of L4/5 intertransverse. RESULTS AND CONCLUSION： At 12 weeks after surgery, bone fusion was found in seven rabbits of the chitosan/calcium- silicate group, four rabbits of the calcium-silicate and chitosan group, and five rabbits of the autologous ilium group. Histological observation showed that the chitosan/calcium-silicate could obviously promote the degradation and osteogenesis. Biomechanical test showed that tensile strength of the chitosan/calcium-silicate group was superior to the other groups （P 〈 0.05）. The results indicated that the three materials can replace autologous bone in lumbar intertransverse fusion, and chitosan/calcium-silicate can promote the process and improve the biomechanical strength of lumbar intertransverse fusion.

关 键 词：壳聚糖/硅酸钙 硅酸钙 壳聚糖 自体骨 脊柱融合 生物材料 

分 类 号：R318[医药卫生—生物医学工程]