新型304-Cu抗菌矫治器的细胞毒性  

Cytotoxicity of a new-type 304-Cu antibiotic stainless orthodontic appliance

作　　者：张丹[1] 任玲[2] 杨柯[2] 张扬[1] 薛楠[1] 

机构地区：[1]中国医科大学口腔医学院正畸科,辽宁省口腔医学研究所正畸教研室,辽宁省沈阳市110002 [2]中国科学院金属研究所,辽宁省沈阳市110016

出　　处：《中国组织工程研究》2012年第25期4622-4626,共5页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省科技厅科学技术计划项目(2010225025);国家自然科学基金项目(2012CB619101);沈阳市科技计划项目(F11-262-9-35)~~

摘　　要：背景:正畸矫治器的戴用破坏了牙齿及其周围组织的环境,造成菌斑堆积,引起一系列不良反应。目的:检测含铜抗菌不锈钢正畸矫治器的细胞毒性。方法:①CCK-8检测细胞增殖:取生长旺盛的人口腔上皮癌细胞KB,实验组加入含铜抗菌不锈钢材料浸提液,对照组加入普通医用不锈钢材料浸提液,阴性对照组加入DMEM培养基,阳性对照组加入苯酚,分别培养24,48,72h。②流式细胞仪Annexin V-FITC/PI双染法检测细胞凋亡:观察普通医用不锈钢、含铜抗菌不锈钢材料对数生长期末人口腔上皮癌细胞KB凋亡的影响,设置空白对照组。结果与结论:①培养48h后,阳性对照组A值随作用时间延长逐渐降低,其余组细胞A值随时间延长逐渐递增(P<0.05),且实验组高于对照组(P<0.05)。普通不锈钢和抗菌不锈钢材料的细胞毒性均为1级。②普通医用不锈钢、含铜抗菌不锈钢组凋亡细胞均较少,且含铜抗菌不锈钢组少于普通医用不锈钢组(P均<0.05)。表明含铜抗菌不锈钢材料生物相容性良好,毒性等级与医用不锈钢相同,符合口腔正畸材料的临床应用要求。BACKGROUND： The wear of orthodontic appliances can destroy the teeth and their surrounding tissue environment, which can result in plaque accumulation and a series of adverse reactions. OBJECTIVE：To detect the cytotoxicity of antibiotic stainless steels orthodontic appliances by CCK-8 and flow cytometry methods. METHODS：（1）The proliferation of cells was detected by CCK-8 method： human oral epithelial carcinoma cells KB which were in vigorous growth were extracted and cultured in 96-well plates. Alloy leaching liquor of the antibacterial stainless steels was added into the experimental group, while the leaching liquor of common stainless steels was added into the control group. The negative control group was added into Dulbecco＇s modified Eagle＇s medium medium, and the positive control group was added into phenol. The cells of each group were cultured for 24, 48 and 72 hours. （2）The apoptotic cells were detected by flow cytometry Annexin V-FITC/PI double staining： the effect of common stainless steels and antibacterial stainless steels materials on the apoptotic of human oral epithelial carcinoma cells KB in the logarithmic phase was investigated. The blank control group was set up. RESULTS and CONCLUSION：（1）After 48 hours of culture, the absorbance value of the positive control group was gradually decreased with the culture time increased; while the absorbance values of other groups were gradually increased with time （P 〈 0.05）, what＇s more, the experimental group was higher than the control group （P 〈 0.05）. The degrees of cytotoxicity in the common stainless steels and antibacterial stainless steels materials were both in grade 1 （2）,The apoptotic cells in common stainless steel group and antibiotic stainless steel group were less than blank control group, besides, the apoptotic cells in the antibiotic stainless steel group were less than those in the common stainless steel group （P 〈 0.05）. These findings suggest that the antibacterial stainless steel h

关 键 词：抗菌不锈钢 正畸矫治器 合金浸提液 细胞毒性 生物材料 

分 类 号：R318[医药卫生—生物医学工程]