退变腰椎Modic改变及分型与肿瘤坏死因子α、基质金属蛋白酶3的表达  

Modic changing and typing of degenerative lumbar and the expression of tumor necrosis factor alpha and matrix metalloproteinase 3

作　　者：姜世峰[1] 申才良[1] 董福龙[1] 张建湘[1] 李勇[1] 

机构地区：[1]安徽医科大学第一附属医院骨科,安徽省合肥市230022

出　　处：《中国组织工程研究》2012年第26期4847-4851,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:大量文献报道了炎性细胞因子在腰椎退变性疾病中发挥着重要的作用,并发现这些退变的腰椎终板及终板下区域椎体在MRI上出现信号的改变。目的:探讨肿瘤坏死因子α、基质金属蛋白酶3与腰椎终板及终板下骨质在MRI上的异常信号改变关系。方法:实验组椎间盘终板标本取自因腰椎退变行腰椎椎间融合患者,共20例;ModicⅠ型、Ⅱ型各10例。对照组椎间盘终板标本取自因腰椎外伤行腰椎椎间融合患者,共10例。应用免疫组织化学染色的方法测定椎间盘终板肿瘤坏死因子α、基质金属蛋白酶3的表达。结果与结论:实验组肿瘤坏死因子α、基质金属蛋白酶3表达水平显著高于对照组(P<0.05),实验组基质金属蛋白酶3,肿瘤坏死因子α在ModicⅠ型终板的表达均明显高于Ⅱ型(P<0.05),实验组中肿瘤坏死因子α与基质金属蛋白酶3的表达无相关性。提示肿瘤坏死因子α、基质金属蛋白酶3可能在腰椎间盘终板退变过程中起促进作用;不同Modic分型肿瘤坏死因子α、基质金属蛋白酶3的表达存在差异。BACKGROUND： In recent years, a large number of reports have shown that in lumbar degenerative diseases, inflammatory cytokines play an important role, and the signals of the degenerative lumbar endplate and regional vertebra endplate are changed on MRI. OBJECTIVE： To investigate the effect of tumor necrosis factor alpha and matrix metalloproteinase 3 expression on Modic changes of lumbar endplate and the bone under endplate on MRI. METHODS： In the experimental group, 20 lumbar endplates were obtained during surgery from the patients with lumbar degeneration treated by lumbar interbody fusion. Both Modic type Ⅰ and Modic type Ⅱ contained 10 cases. As the control group, 10 lumbar endplates were also got during surgery from patients with trauma of vertebra treated with lumbar interbody fusion. The expression of tumor necrosis factor alpha and matrix metalloproteinase 3 in lumbar endplate was tested by immunohistochemical staining. RESULTS AND CONCLUSION： The positive ratio of tumor necrosis factor alpha and matrix metalloproteinase 3 in the experimental group was significantly higher than that in the control group （P 〈 0.05）; the expressions of tumor necrosis factor alpha and matrix metalloproteinase 3 in Modic type Ⅰ were significantly higher than those in Modic type Ⅱ （P 〈 0.05）; in the experimental group, the expression of matrix metalloproteinase 3 showed no correlation with the expression of tumor necrosis factor alpha. The expression of tumor necrosis factor alpha and matrix metalloproteinase 3 may promote vertebra endplates degeneration; there was significant difference of the tumor necrosis factor alpha and matrix metalloproteinase 3 expression between different Modic types.

关 键 词：椎间盘退变 基质金属蛋白酶3 肿瘤坏死因子Α 核磁共振成像 Modic分型 

分 类 号：R318[医药卫生—生物医学工程]